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Brilliant Violet 421™ anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
5.1H11 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1, NCAM-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5.1H11_BV421_CD56_Antibody_FC_081315
Human peripheral blood lymphocytes stained with CD56 (clone 5.1H11) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • 5.1H11_BV421_CD56_Antibody_FC_081315
    Human peripheral blood lymphocytes stained with CD56 (clone 5.1H11) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
362551 25 tests 146€
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362552 100 tests 322€
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human myotube cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References
  1. Walsh FS, et al. 1981. Nature 289:60. (FC)
  2. Pavlath GK, et al. 1986. J. Cell Biol. 102:124. (FC)
  3. Pavlath GK, et al. 1989. Nature 337:570. (FC)
  4. Pulido R, et al. 1988. J. Immunol. 140:3851. (FC)
Product Citations
  1. Estep BK, et al. 2022. iScience. 25:105544. PubMed
  2. Zhu X, et al. 2023. Front Immunol. 14:1114802. PubMed
  3. Lellahi SM, et al. 2023. Front Immunol. 13:1058963. PubMed
  4. Lukas H, et al. 2023. National Library of Medicine. . 120(33): e2300343120. PubMed
  5. Heger L, et al. 2018. Front Immunol. 9:744. PubMed
  6. Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
  7. Ziegler CGK, et al. 2021. Cell. 184(18):4713-4733.e22. PubMed
  8. Thiel BA, et al. 2021. PLoS One. 16:e0249477. PubMed
  9. Grant R, et al. 2021. Methods Protoc. 4:. PubMed
  10. Aguilar–Briseñ o JA, et al. 2020. Nat Commun. 2.664583333. PubMed
RRID
AB_2566060 (BioLegend Cat. No. 362551)
AB_2566060 (BioLegend Cat. No. 362552)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparan sulfate
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD56 (NCAM) Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD56 (NCAM) 5.1H11 FC
APC anti-human CD56 (NCAM) 5.1H11 FC
PerCP/Cyanine5.5 anti-human CD56 (NCAM) 5.1H11 FC
PE anti-human CD56 (NCAM) 5.1H11 FC
PE/Cyanine7 anti-human CD56 (NCAM) 5.1H11 FC
APC/Cyanine7 anti-human CD56 (NCAM) 5.1H11 FC
PE/Cyanine5 anti-human CD56 (NCAM) 5.1H11 FC
Alexa Fluor® 647 anti-human CD56 (NCAM) 5.1H11 FC
Alexa Fluor® 488 anti-human CD56 (NCAM) 5.1H11 FC
Pacific Blue™ anti-human CD56 (NCAM) 5.1H11 FC
Alexa Fluor® 700 anti-human CD56 (NCAM) 5.1H11 FC
PerCP anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 650™ anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 510™ anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 421™ anti-human CD56 (NCAM) 5.1H11 FC
Biotin anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 605™ anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 570™ anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 711™ anti-human CD56 (NCAM) 5.1H11 FC
PE/Dazzle™ 594 anti-human CD56 (NCAM) 5.1H11 FC
FITC anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 785™ anti-human CD56 (NCAM) 5.1H11 FC
Ultra-LEAF™ Purified anti-human CD56 (NCAM) 5.1H11 FC
APC/Fire™ 750 anti-human CD56 (NCAM) 5.1H11 FC
PE anti-human CD56 (NCAM) 5.1H11 FC
APC anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 750™ anti-human CD56 (NCAM) 5.1H11 FC
TotalSeq™-A0047 anti-human CD56 (NCAM) 5.1H11 PG
TotalSeq™-C0047 anti-human CD56 (NCAM) 5.1H11 PG
TotalSeq™-B0047 anti-human CD56 (NCAM) 5.1H11 PG
Spark NIR™ 685 anti-human CD56 (NCAM) 5.1H11 FC
KIRAVIA Blue 520™ anti-human CD56 (NCAM) 5.1H11 FC
GMP PE anti-human CD56 (NCAM) 5.1H11 FC
TotalSeq™-D0047 anti-human CD56 (NCAM) 5.1H11 PG
GMP APC anti-human CD56 (NCAM) 5.1H11 FC
Spark YG™ 593 anti-human CD56 (NCAM) 5.1H11 FC
Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD56 (NCAM) SF 5.1H11 FC
Spark Red™ 718 anti-human CD56 (NCAM) 5.1H11 FC
Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF 5.1H11 FC
APC/Fire™ 810 anti-human CD56 (NCAM) Antibody 5.1H11 FC
Go To Top Version: 1    Revision Date: 08-13-2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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