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Brilliant Violet 421™ anti-mouse Allergin-1 Antibody

Pricing & Availability
Clone
TX83 (See other available formats)
Regulatory Status
RUO
Other Names
Allergy inhibitory receptor 1, mast cell Ag-32, MCA-32, Milr1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TX83_BV-421_-Allergin-1_FC_082024
C57BL/6 mouse peritoneal cavity cells were stained with anti-mouse c-kit (clone ACK2) Brilliant Violet 785™, anti-anti-mouse FcεRIα (clone MAR-1) APC and with anti-mouse Allergin-1 (clone TX83) Brilliant Violet 421™ (left) or mouse IgG1, κ Brilliant Violet 421™ isotype control (right). Data shown is gated on the c-kit+ population.
  • TX83_BV-421_-Allergin-1_FC_082024
    C57BL/6 mouse peritoneal cavity cells were stained with anti-mouse c-kit (clone ACK2) Brilliant Violet 785™, anti-anti-mouse FcεRIα (clone MAR-1) APC and with anti-mouse Allergin-1 (clone TX83) Brilliant Violet 421™ (left) or mouse IgG1, κ Brilliant Violet 421™ isotype control (right). Data shown is gated on the c-kit+ population.
See Brilliant Violet 421™ spectral data
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141809 50 µg 216€
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Description

Allergin-1, also known as mast cell Ag-32, is a 60 kD single pass transmembrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain in the extracellular region and one immunoreceptor tyrosine-based inhibitory motif (ITIM)-like domain in the cytoplasmic region. Allergin-1 is expressed on mast cells, dendritic cells, and macrophages as well as at low levels on neutrophils. After tyrosine phosphorylation, allergin-1 recruits PTPN6, PTPN11, and INPP5D, which results in a diminution of IgE-mediated degranulation in mast cells. No ligands have been identified for this molecule.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Transfected cells expressing allergin-1-Flag
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Inhibits FcεRI-mediated degranulation in bone marrow-derived cultured mast cells1. Additional reported applications (for the relevant formats) include: blocking1.

Application References
  1. Hitomi K, et al. 2010. Nat. Immunol. 11:601. (Block)

Antigen Details

Structure
Single pass transmembrane protein of 60 kD; contains one Ig-like C2-like domain and one ITIM-like domain
Distribution

Mast cells, dendritic cells, macrophages, neutrophils

Function
Inhibits IgE-mediated degranulation of mast cells
Interaction
PTPN6, PTPN11 and INPP5D
Cell Type
Dendritic cells, Macrophages, Mast cells, Neutrophils
Biology Area
Cell Biology, Immunology, Signal Transduction
Antigen References

1. Hitomi K, et al. 2010. Nat. Immunol. 11:601.
2. Pirozzi G, et al. 1995. J. Immunol. 155:5811.

Gene ID
380732 View all products for this Gene ID
UniProt
View information about Allergin-1 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 08-12-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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