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Brilliant Violet 421™ anti-mouse/human Ki-67 Antibody

Pricing & Availability
Clone
11F6 (See other available formats)
Regulatory Status
RUO
Other Names
Mki67, Ki67, Ki-67, MIB-1, KIA
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
11F6_BV421_Ki-67_Antibody_010419
Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 11F6) Alexa Fluor® 647 (filled histogram) or rat IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
  • 11F6_BV421_Ki-67_Antibody_010419
    Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 11F6) Alexa Fluor® 647 (filled histogram) or rat IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
  • 11F6_BV421_Ki-67_Antibody_3_010819_ICFC
    Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 11F6) Brilliant Violet 421™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
  • 11F6_BV421_Ki67_Antibody_IHCF_090216
    C57BL/6 frozen intestine section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat serum for one hour at room temperature. Then the section was stained with 2.5 µg/ml of Ki67 (clone 11F6) Brilliant Violet 421™ (blue) and 5 µg/ml of CD324 (clone DECMA-1) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DRAQ5™ (green). The image was captured with a 10X objective.
Compare all formats See Brilliant Violet 421™ spectral data See high resolution IHC-F data...
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151208 100 µg 340€
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Description

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular  immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  2. Bieniasz–Krzywiec P, et al. 2020. Cell Metabolism. 30(5):917-936. PubMed
  3. Burns JC, et al. 2020. eLife. 9:00. PubMed
  4. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  5. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  6. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
RRID
AB_2629748 (BioLegend Cat. No. 151208)

Antigen Details

Structure
325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
Distribution

Nucleus and chromosomes.

Function
Required for cell cycle progression and proliferation.
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References
  1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
  2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
  3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
  4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
  5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
  6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID
4288 View all products for this Gene ID 17345 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 01-08-2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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