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Brilliant Violet 605™ anti-mouse CD86 Antibody

Pricing & Availability
Clone
GL-1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-2, B70, Ly-58
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
GL-1_BV605_033012
LPS-stimulated (3 days) C57BL/6 mouse splenocytes were stained with GL-1 Brilliant Violet 605™ (filled histogram) or rat IgG2a, κ Brilliant Violet 605™ isotype control (open histogram).
  • GL-1_BV605_033012
    LPS-stimulated (3 days) C57BL/6 mouse splenocytes were stained with GL-1 Brilliant Violet 605™ (filled histogram) or rat IgG2a, κ Brilliant Violet 605™ isotype control (open histogram).
Compare all formats See Brilliant Violet 605™ spectral data
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105037 125 µL 181€
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS-activated CBA/Ca mouse splenic B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).

Application References
  1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
  2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
  5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
  6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  9. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
  11. de Verteuil DA, et al. 2014. J Immunol. 193:1121. PubMed
Product Citations
  1. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
  2. Cui B, et al. 2023. Int Immunopharmacol. 114:109541. PubMed
  3. Tunali G, et al. 2023. J Clin Invest. :. PubMed
  4. Pagni RL, et al. 2022. Front Immunol. 13:1005937. PubMed
  5. Barberio AE, et al. 2023. Bioeng Transl Med. 8:e10453. PubMed
  6. Zeng S, et al. 2023. Front Oncol. 13:1171926. PubMed
  7. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
  8. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
  9. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
  10. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  11. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  12. Barberio AE, et al. 2020. ACS Nano. 14:11238. PubMed
  13. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  14. Duan L, et al. 2021. Immunity. 54:2256. PubMed
  15. Kato Y, et al. 2020. Immunity. 53(3):548-563. PubMed
  16. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  17. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  18. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
  19. Liu D, et al. 2020. J Exp Med. 217:. PubMed
  20. Hamilton J, et al. 2015. J Immunol. 194:5022. PubMed
  21. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  22. Guilliams M, et al. 2022. Cell. 185:379. PubMed
  23. Ishikura N, et al. 2022. Oncol Rep. 47:. PubMed
  24. Zhang X, et al. 2020. Endocr Relat Cancer. 27:469. PubMed
  25. Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed
  26. Škrnjug I, et al. 2014. PLoS One. 9:95728. PubMed
  27. Macal M et al. 2018. Immunity. 48(4):730-744 . PubMed
  28. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  29. Hardy C, et al. 2013. J Immunol. 191:5278. PubMed
  30. Ceglia V, et al. 2021. J Immunol. 207:2060. PubMed
  31. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  32. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
RRID
AB_11204429 (BioLegend Cat. No. 105037)

Antigen Details

Structure
Ig superfamily, 80 kD
Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Function
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
12524 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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