- Clone
- Poly4054 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Goat Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
405430 | 50 µg | 229€ |
Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.
- Application References
- RRID
-
AB_2687100 (BioLegend Cat. No. 405430)
Related FAQs
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC Goat anti-rat IgG (minimal x-reactivity)
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FITC Goat anti-rat IgG (minimal x-reactivity)
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HRP Goat anti-rat IgG (minimal x-reactivity)
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PE Goat anti-rat IgG (minimal x-reactivity)
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Purified Goat anti-rat IgG (minimal x-reactivity)
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PE/Cyanine7 Goat anti-rat IgG (minimal x-reactivity)
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Cyanine3 Goat anti-rat IgG (minimal x-reactivity)
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Brilliant Violet 421™ Goat anti-rat IgG (minimal x-reactivity)
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Alexa Fluor® 647 Goat anti-rat IgG (minimal x-reactivity)
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Alexa Fluor® 488 Goat anti-rat IgG (minimal x-reactivity)
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Alexa Fluor® 555 Goat anti-rat IgG (minimal x-reactivity)
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Alexa Fluor® 594 Goat anti-rat IgG (minimal x-reactivity)
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PerCP/Cyanine5.5 Goat anti-rat IgG (minimal x-reactivity)
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APC/Fire™ 750 Goat anti-rat IgG (minimal x-reactivity)
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Brilliant Violet 605™ Goat anti-rat IgG (minimal x-reactivity)
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PE/Dazzle™ 594 Goat anti-rat IgG (minimal x-reactivity)
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Biotin Goat anti-rat IgG (minimal x-reactivity)
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