Brilliant Violet 650™ anti-mouse CD279 (PD-1) Antibody, CD279, 29F.1A12

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Clone: 29F.1A12 (See other available formats)
Regulatory Status: RUO
Other Names: PD-1, Programmed Death-1, PDCD1
Isotype: Rat IgG2a, κ
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Product Citations: publications

29Fdot1A12_BV650_CD279_Antibody_070224 — Con-A and IL-2 stimulated C57BL/6 mouse splenocytes (3 days) were stained with anti-mouse CD3 (clone 145-2C11) FITC and anti-mouse CD279 (PD-1) (clone 29F.1A12) Brilliant Violet 650™ (left) or rat IgG2a, κ Brilliant Violet 650™ isotype control (right).

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135243

50 µg

128€

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Description

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: PD-1 cDNA followed by PD-1-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue³, in vivo blocking of PD-1 binding to its ligands^2,3, and spatial biology (IBEX)^5,6.
RRID: AB_3662377 (BioLegend Cat. No. 135243)

Antigen Details

Structure: A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.
Distribution: Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.
Function: Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.
Ligand/Receptor: B7-H1 (PD-L1) and B7-DC (PD-L2)
Cell Type: B cells, T cells
Biology Area: Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2008. J Immunol. 181:5313
Gene ID: 18566 View all products for this Gene ID
UniProt: View information about CD279 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD279 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse CD279 (PD-1)	29F.1A12	FC
Purified anti-mouse CD279 (PD-1)	29F.1A12	FC,IHC-F,Block,ELISA
PerCP/Cyanine5.5 anti-mouse CD279 (PD-1)	29F.1A12	FC
APC anti-mouse CD279 (PD-1)	29F.1A12	FC
Biotin anti-mouse CD279 (PD-1)	29F.1A12	FC
FITC anti-mouse CD279 (PD-1)	29F.1A12	FC
PE/Cyanine7 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 421™ anti-mouse CD279 (PD-1)	29F.1A12	FC,SB
Brilliant Violet 605™ anti-mouse CD279 (PD-1)	29F.1A12	FC
APC/Cyanine7 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 785™ anti-mouse CD279 (PD-1)	29F.1A12	FC
PE/Dazzle™ 594 anti-mouse CD279 (PD-1)	29F.1A12	FC
Alexa Fluor® 647 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 711™ anti-mouse CD279 (PD-1)	29F.1A12	FC
GoInVivo™ Purified anti-mouse CD279 (PD-1)	29F.1A12	FC
APC/Fire™ 750 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 510™ anti-mouse CD279 (PD-1)	29F.1A12	FC
Ultra-LEAF™ Purified anti-mouse CD279 (PD-1)	29F.1A12	FC,IHC-F,Block
APC/Fire™ 810 anti-mouse CD279 (PD-1) Antibody	29F.1A12	FC
PE/Fire™ 810 anti-mouse CD279 (PD-1) Antibody	29F.1A12	FC
PE/Cyanine5 anti-mouse CD279 (PD-1)	29F.1A12	FC
PE/Fire™ 640 anti-mouse CD279 (PD-1)	29F.1A12	FC
Spark Red™ 718 anti-mouse CD279 (PD-1)	29F.1A12	FC
PerCP/Fire™ 806 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 750™ anti-mouse CD279 (PD-1)	29F.1A12	FC
PerCP/Fire™ 780 anti-mouse CD279 (PD-1)	29F.1A12	FC
PE/Fire™ 700 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 650™ anti-mouse CD279 (PD-1)	29F.1A12	FC
Spark Blue™ 574 anti-mouse CD279 (PD-1) (Flexi-Fluor™)	29F.1A12	FC
Spark PLUS B550™ anti-mouse CD279 (PD-1)	29F.1A12	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07-02-2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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