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- Other Names
- Bone Morphogenetic Protein 7, Osteogenic Protein 1, OP-1, OP1, BMP7
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- Product Citations
- publications
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Cell Vive™ GMP recombinant human BMP-7 induces alkaline phosphatase (ALP) production in the mouse chrondrogenic cell line ATDC5 in a dose dependent manner. The ED50 for this effect is 18 – 72 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 795715 | 50 µg | 840€ | ||||
Bone morphogenetic proteins (BMPs) are multi-functional growth factors that belong to the transforming growth factor beta (TGF-beta) superfamily. BMPs play a key role in embryonic development, especially during heart, neural and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptor and type II/IIB activin receptors. Initially identified as an inducer of ectopic bone and cartilage, BMP-7 is a homodimer. It is known BMP-7 can form heterodimers with BMP-2 and BMP-4 with stronger activity than the homodimer, and those heterodimers have a predominant function in early stages of embryonic mouse development. BMP-7 was recognized as an anti-fibrotic molecule, antagonizing TGF-β1. Fibrosis is associated with the emergence of fibroblasts derived from endothelial cells, suggesting an endothelial-mesenchymal transition (EndMT). TGF-β1 induces endothelial cells to undergo EndMT, whereas bone BMP-7 preserved the endothelial phenotype. BMP-7 attenuates liver fibrosis via regulation EGFR. BMP-7 inhibits fibrosis in the kidney, lung, liver, heart, peritoneum, oral submucosa tissue, and colonic wall. BMP-7 is associated to gastric cancer, lung cancer, and melanoma.
Product DetailsBioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
- Low endotoxin level (≤ 0.1 EU/µg)
- Purity (≥ 95% or higher)
- Bioburden testing
- Mycoplasma testing
- Batch-to-batch consistency
- Vendor qualification
- Raw material traceability and documentation
- Documented procedures and employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Product Details
- Source
- Human BMP-7, amino acid (Ser293–His431) (Accession No. P18075), was expressed in CHO cells.
- Molecular Mass
- The 139 amino acid recombinant protein has a predicted molecular mass of approximately 15.68 kD. The DTT-reduced and non-reduced protein migrates at approximately 20 kD and 40 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Ser.
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N-terminal
Sequence Analysis - Ser-Thr-Gly-Ser-Lys-Gln-Arg-Ser-Gln-Asn
- Purity
- ≥ 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- Protein was lyophilized from 0.22 µm filtered solution containing 0.1% TFA and 5% trehalose
- Endotoxin Level
- Less than or equal to 0.1 EU per µg of protein as determined by LAL method.
- Residual Host Cell Protein Content
- ≤ 0.500 ng/µg by ELISA
- Concentration
- 50 µg size is lyophilized
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile 4 mM HCL . Before reconstitution, make sure sterile 4 mM HCL and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (4 mM HCL) to the vial, to obtain the recommended stock concentration 250 µg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all of the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 µg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/ thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months.
- Activity
- Human BMP-7 induces Alkaline Phosphatase (ALP) in the mouse chondrogenic cell line ATDC5 in a dose-dependent manner. The ED50 for this effect is 18 – 72 ng/mL.
- Application
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Bioassay
- Application Notes
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Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Disclaimer
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BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Antigen Details
- Structure
- Heterodimer
- Function
- Embryonic development and adult homeostasis
- Interaction
- Osteoblasts, Langerhans cells, adipocytes, endothelial cells, macrophages, CD4 T cells, rheumatoid synoviocytes
- Ligand/Receptor
- Type I receptors: BMP-RIA (ALK3), BMP-RIB (ALK6), and Activin RI (ALK2) Type II receptors: BMP-RII, Act-RIIA, and Act-RIIB
- Bioactivity
- BMP-7 induces alkaline phosphatase production in the mouse chondrogenic cell line ATDC5.
- Cell Sources
- Human cancer cells, rheumatoid fibroblast-like synoviocytes
- Antigen References
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- Chen J, et al. 2010. Anticancer Res. 30:1113-20.
- Aoki M, et al. 2010. Br J Cancer. 104:714.
- Neugebauer JM, et al. 2015. Proc Natl Acad Sci U S A. 112:E2307-16.
- Varas A, et at. 2015. Arthritis Res Ther. 17:192.
- Reible B, et al. 2018. Int J Mol Sci. 19:1674.
- Kim HS, et al. 2019. eLife 8:e48872.
- Cortez MA, et al. 2020. Nat Commun. 11:4840.
- Gene ID
- 655 View all products for this Gene ID
- UniProt
- View information about BMP-7 on UniProt.org
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