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FITC anti-human CD53 Recombinant Antibody

Pricing & Availability
Clone
QA19A07 (See other available formats)
Regulatory Status
RUO
Other Names
TSPAN25
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA19A07_FITC_CD53_Recombinant_Antibody_070121.png
Human peripheral blood lymphocytes were stained with anti-human CD53 recombinant (clone QA19A07) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
  • QA19A07_FITC_CD53_Recombinant_Antibody_070121.png
    Human peripheral blood lymphocytes were stained with anti-human CD53 recombinant (clone QA19A07) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
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325703 25 tests 81€
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325704 100 tests 141€
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Description

CD53 is a 35-42 kD type III tetraspan membrane protein. It is expressed on all leukocytes, but not on platelets or erythrocytes. CD53 is thought to be involved in signal transduction. It has been shown to interact with a number of proteins including IL-4, CD20, CD2, CD9, IL-2, VLA-4, and CD4. Cross-linking of CD53 promotes B cell activation. The CD53 antigen is highly glycosylated and treatment with endoglycosidase F reduces the apparent molecular weight of this protein by approximately 25 kD. The HI29 antibody has been shown to be useful for flow cytometry, and immunohistochemistry (frozen).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
RRID
AB_2894472 (BioLegend Cat. No. 325703)
AB_2894472 (BioLegend Cat. No. 325704)

Antigen Details

Structure
Cell surface protein, tetraspan family, four membrane spanning hydrophobic domains, 35-42 kD.
Distribution

Hematopoietic cells including neutrophils, monocytes, B cells, T cells (single positive thymocytes and peripheral T cells), and eosinophils. Not expressed on platelets, erythrocytes, and non-hematopoietic cells.

Function
Signal transduction, cross-linking on B cells causes activation.
Interaction
IL-4, CD20, CD2, CD9, IL-2, integrin α4, integrin β1, CD4
Ligand/Receptor
NK cell, B cells, Neutrophils, Eosinophils
Antigen References
  1. Amiot M. 1990. J Immunol. 145:4322-5.
  2. Angelisova P, et al. 1990. Immunogenetics. 32:281-5.
  3. Olweus J, et al. 1993. J Immunol. 153:4997.
  4. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford: Oxford University Press.
Gene ID
963 View all products for this Gene ID
UniProt
View information about CD53 on UniProt.org
Go To Top Version: 1    Revision Date: 07-01-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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