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FITC anti-human CD77 Antibody

Pricing & Availability
Clone
5B5 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD77.5
Other Names
globotriaosylcermamide (Gb3), Burkitt's lymphoma associated antigen (BLA), cermadie trihexoside (CTH), and Pk blood group antigen
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
5B5_FITC_CD77_Antibody_FC_052413
Human B-cell Burkitt's lymphoma cell line Daudi was stained with CD77 (clone 5B5) FITC (filled histogram) or mouse IgM, κ FITC isotype control (open histogram).
  • 5B5_FITC_CD77_Antibody_FC_052413
    Human B-cell Burkitt's lymphoma cell line Daudi was stained with CD77 (clone 5B5) FITC (filled histogram) or mouse IgM, κ FITC isotype control (open histogram).
See FITC spectral data
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357103 25 tests 81€
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357104 100 tests 202€
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Description

CD77 is also known as Gb3 glycosphingolipid. It was found specifically expressed on Burkitt's lymphoma cells and on a subset of germinal center B lymphocytes. CD77 has been described as a receptor for Shiga Toxin and Verotoxin 1 (VT1), which is produced by Shigella dysenteriae and some strains of E. coli. It is used as a marker for germinal center stage B-cells. CD19 has been reported as a ligand for CD77 and their interaction functions in adhesion and signal transduction at a specific stage of B cell development. Studies have shown that cross-linking CD77 induces apoptosis in some B cell lines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral blood mononuclear cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for relevant formats) include:  Western blotting1 and immunohistochemical staining of frozen tissue sections1.

Application References
  1. Fyfe G, et al. 1987. J. Immunol. 139:2187. (WB, IHC)
Product Citations
  1. Maldonado-Báez L, et al. 2013. J Cell Biol. 233:201. PubMed
  2. Mohamed LA, et al. 2019. Exp Neurol. 27:316. PubMed
  3. Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed
RRID
AB_2562160 (BioLegend Cat. No. 357103)
AB_2562160 (BioLegend Cat. No. 357104)

Antigen Details

Structure
Glycosphingolipid
Distribution

Burkitt's lymphoma cells, a subset of germinal center B lymphocytes

Function
Involved in cell adhesion and signaling transduction by binding CD19
Ligand/Receptor
Receptor of Shiga Toxin and Verotoxin 1 (VT1) and CD19
Cell Type
B cells, Lymphocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Tétaud C, 2003. J. Biol. Chem. 278:45200.
2. Maloney MD, 1994. J. Exp. Med. 1994 180:191.
3. Mangeney M, et al. 1991. Eur. J. Immunol. 21:1131.
4. Mangeney M, et al. 1993. Cancer Res. 53:5314.

Gene ID
NA
Go To Top Version: 2    Revision Date: 01-27-2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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