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FITC anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
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Product Citations
publications
RM4-5
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) FITC (solid line) or rat IgG2a, κ FITC isotype control (dotted line).
  • RM4-5
    C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) FITC (solid line) or rat IgG2a, κ FITC isotype control (dotted line).
Compare all formats See FITC spectral data
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100509 50 µg 24€
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100510 500 µg 80€
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  2. Xu C, et al. 2017. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.05.071. PubMed
  3. Wang L, et al. 2018. Exp Ther Med. 15:1532. PubMed
  4. Yu HH, et al. 2021. J Am Heart Assoc. 10:e023077. PubMed
  5. Aimaitijiang A, et al. 2022. Genes Cells. 27:25. PubMed
  6. Yu C, et al. 2022. Invest Ophthalmol Vis Sci. 63:1. PubMed
  7. Piñeros AR, et al. 2022. Cell Rep. 39:111011. PubMed
  8. Chen C, et al. 2022. Sci Adv. 8:eabo4363. PubMed
  9. Liu P, et al. 2023. Clin Immunol. 246:109212. PubMed
  10. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  11. Peng Q, et al. 2023. EMBO Rep. 24:e56034. PubMed
  12. Takacs GP, et al. 2023. Front Immunol. 13:993444. PubMed
  13. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  14. Stone VM, et al. 2022. iScience. 25:105070. PubMed
  15. Akhtar MN, et al. 2022. MBio. 13:e0330921. PubMed
  16. Cheng C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:261. PubMed
  17. Chen Y, et al. 2022. Am J Cancer Res. 12:5286. PubMed
  18. Han X, et al. 2022. Nutrients. 14:. PubMed
  19. Gao X, et al. 2023. Front Immunol. 14:1142428. PubMed
  20. Qin T, et al. 2023. Virol J. 20:75. PubMed
  21. Zhang YT, et al. 2023. Front Immunol. 14:1085456. PubMed
  22. Wang X, et al. 2023. NPJ Vaccines. 8:76. PubMed
  23. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  24. Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed
  25. Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed
  26. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  27. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  28. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  29. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  30. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  31. Gilleron J et al. 2018. Cell reports. 25(12):3329-3341 . PubMed
  32. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 5:64. PubMed
  33. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  34. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  35. Li ZX, et al. 2022. Arch Virol. :. PubMed
  36. Zhang S, et al. 2022. Cell Death Dis. 13:844. PubMed
  37. Abou-Hamad J, et al. 2022. iScience. 25:105524. PubMed
  38. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  39. Zheng H, et al. 2021. Frontiers in Cell and Developmental Biology. 9:641527. PubMed
  40. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  41. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  42. Ogawa M, et al. 2019. J Biol Methods. 5:e102. PubMed
  43. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
  44. Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed
  45. Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
  46. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  47. Jin X, et al. 2019. Mol Cell. 73:22:00. PubMed
  48. Sehnert B, et al. 2022. Front Immunol. 13:921488. PubMed
  49. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  50. Yi M, et al. 2021. J Hematol Oncol. 14:27. PubMed
  51. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  52. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  53. Zhang C, et al. 2021. Clin Transl Immunology. 10:e1310. PubMed
  54. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  55. Forni MF, et al. 2021. Front Oncol. 11:667715. PubMed
  56. Sundberg T, et al. 2014. Proc Natl Acad Sci U S A. 111:12468. PubMed
  57. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  58. Graham DB, et al. 2018. Nat Med. 24:1762. PubMed
  59. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  60. Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed
  61. Qi X, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.25. PubMed
  62. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  63. Jackstadt R, et al. 2019. Cancer Cell. 36:319. PubMed
  64. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  65. Gu Y, et al. 2021. Cell Res. Online ahead of print. PubMed
  66. Spinner C, et al. 2015. Sci Rep. 5:16269. PubMed
  67. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  68. Gozgit JM, et al. 2021. Cancer Cell. :. PubMed
  69. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  70. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  71. Duan RN, et al. 2021. J Neuroinflammation. 18:145. PubMed
  72. Repáraz D, et al. 2022. J Immunother Cancer. 10:. PubMed
  73. Zhang YN, et al. 2020. EBioMedicine. 56:102819. PubMed
  74. Sebastian M, et al. 2016. J Immunol. 196: 144 - 155. PubMed
  75. Wan X, et al. 2018. Nature. 560:107. PubMed
  76. Wheeler MA, et al. 2019. Cell. 176:581. PubMed
  77. Fang E, et al. 2022. MedComm (2020). 3:e117. PubMed
  78. Belgiovine C, et al. 2021. Eur J Immunol. 51:2677. PubMed
  79. Takeshita Y, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  80. Kulkarni R, et al. 2021. PLoS One. 16:e0257191. PubMed
  81. Lischke T, et al. 2013. Infect Immun . 81:4091. PubMed
  82. Alhosaini K, et al. 2021. Brain Sci. :11. PubMed
  83. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  84. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  85. Anger-Góra N, et al. 2021. Oncol Lett. 22:582. PubMed
  86. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  87. Chou T, et al. 2016. Nat Commun. 7:11904. PubMed
  88. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  89. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  90. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  91. Aguilera M, et al. 2021. Int J Mol Sci. 22:. PubMed
  92. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  93. Kyburz A, et al. 2017. Clin Exp Allergy. 47:1331. PubMed
  94. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  95. Chen C, et al. 2021. J Virol. 95:e0082921. PubMed
  96. Tu J, et al. 2022. Mol Ther Nucleic Acids. 27:733. PubMed
  97. Zhou X, et al. 2021. Cell Reports. 34(1):108601. PubMed
  98. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  99. Takenori Inomata, Jing Hua, Antonio Di Zazzo 2016. Sci Rep. 6:39924. PubMed
  100. Kim M, et al. 2018. Immunity. 49:151. PubMed
  101. Papa I, et al. 2017. Nature. 547:318. PubMed
  102. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  103. Zhang J, et al. 2018. Nature. 553:91. PubMed
  104. Dong M, et al. 2022. J Immunother Cancer. 10:. PubMed
  105. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  106. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  107. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  108. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  109. Uribe-Herranz M, et al. 2018. JCI Insight. 3. PubMed
  110. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  111. Bao Z, et al. 2020. Ann Transplant. 25:e921287. PubMed
  112. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  113. Bell CR, et al. 2022. Nat Commun. 13:2063. PubMed
  114. de Assis LVM, et al. 2022. Commun Biol. 5:461. PubMed
  115. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  116. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  117. Lu J, et al. 2022. Int J Mol Med. 49:. PubMed
  118. Harrison DK, et al. 2021. Transplantation. 105:540. PubMed
  119. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  120. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  121. Leon-Ponte M, et al. 2006. Blood. 109:3139. PubMed
  122. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  123. Yeon S, et al. 2017. Sci Rep. 10.1038/s41598-017-11492-7. PubMed
  124. Wang X, et al. 2022. Elife. 11:. PubMed
  125. Helfricht A, et al. 2020. J Exp Med. 217:00:00. PubMed
  126. Ren D, et al. 2020. Am J Cancer Res. 2851:10. PubMed
  127. Wheeler MA, et al. 2020. Nature. 578:593. PubMed
  128. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  129. Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
  130. Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
  131. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
  132. Campisi L, et al. 2016. Nat Immunol. 10.1038/ni.3512. PubMed
  133. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
  134. Siegmund K, et al. 2011. J Immunol. 186:3452. PubMed
  135. Shi GN, et al. 2021. Ther Adv Med Oncol. 13:1758835920987056. PubMed
  136. Ren X, et al. 2021. Cell Death Dis. 12:484. PubMed
  137. Rana MN, et al. 2021. Front Pharmacol. 12:643215. PubMed
RRID
AB_312712 (BioLegend Cat. No. 100509)
AB_312712 (BioLegend Cat. No. 100510)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 3    Revision Date: 01-29-2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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