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FITC anti-mouse CD48 Antibody

Pricing & Availability
Clone
HM48-1 (See other available formats)
Regulatory Status
RUO
Other Names
BCM1, SLAMF2, Blast-1
Isotype
Armenian Hamster IgG
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Product Citations
publications
HM48-1
C57BL/6 splenocytes stained with HM48-1 FITC
  • HM48-1
    C57BL/6 splenocytes stained with HM48-1 FITC
Compare all formats See FITC spectral data
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103403 50 µg 85€
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103404 500 µg 244€
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Description

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse T lymphoma MBL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References
  1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
  2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
Product Citations
  1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  2. Lengefeld J, et al. 2021. Sci Adv. 7:eabk0271. PubMed
  3. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  4. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  5. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  6. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  7. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed
  8. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  9. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  10. Guo B, et al. 2018. Nat Med. 24:360. PubMed
  11. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  12. Ganuza M, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.04.006. PubMed
  13. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  14. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  15. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  16. Georgievski A, et al. 2022. Cell Death Dis. 13:337. PubMed
  17. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  18. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  19. Dawar S, et al. 2016. Cell Death Dis. 7:e2509. PubMed
  20. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  21. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  22. Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed
  23. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  24. Nakamura-Ishizu A, et al. 2012. Blood. 119:5429. PubMed
  25. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  26. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  27. Artinger E, et al. 2013. Proc Natl Acad Sci U S A. 110:12000. PubMed
  28. Theocharides A, et al. 2012. J Exp Med. 209:1883. PubMed
  29. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  30. Koide S, et al. 2022. iScience. 25:103603. PubMed
  31. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  32. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  33. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  34. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  35. Lv K, et al. 2021. Cell Stem Cell. 28(7):1275-1290.e9. PubMed
  36. Chou S, et al. 2013. Exp Hematol. 41:479. PubMed
  37. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  38. Abdel Malik R, et al. 2017. Circ Res. 120:99. PubMed
RRID
AB_313018 (BioLegend Cat. No. 103403)
AB_313018 (BioLegend Cat. No. 103404)

Antigen Details

Structure
Ig superfamily, 45 kD
Distribution

T cells and B cells, monocytes/macrophages

Function
Adhesion, T cell recognition
Ligand/Receptor
CD2, CD244
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
12506 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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