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FITC anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_FITC_011409.jpg
C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
  • HK1dot4_FITC_011409.jpg
    C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
Compare all formats See FITC spectral data
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128005 50 µg 72€
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128006 500 µg 235€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  2. de la Rosa X, et al. 2018. Am J Pathol. 188:950. PubMed
  3. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  4. Duan Y, et al. 2019. Circ Res. 125:969. PubMed
  5. Liang J, et al. 2021. Cancer Manag Res. 13:6977. PubMed
  6. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  7. Fang C, et al. 2022. J Pathol. 256:310. PubMed
  8. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  9. Du Y, et al. 2022. FASEB J. 36:e22248. PubMed
  10. Helfen A, et al. 2022. Neoplasia. 28:100792. PubMed
  11. Liu X, et al. 2022. Adv Sci (Weinh). 9:e2105650. PubMed
  12. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  13. Campisi L, et al. 2022. Nature. 606:945. PubMed
  14. Silva Pereira S, et al. 2022. Elife. 11: . PubMed
  15. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  16. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  17. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  18. Soni N, et al. 2022. FASEB Bioadv. 4:798. PubMed
  19. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  20. Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed
  21. Meng S, et al. 2023. Stem Cell Investig. 10:2. PubMed
  22. Hettwer J, et al. 2022. Cardiovasc Res. 118:2778. PubMed
  23. Fernández-García V, et al. 2022. Biomed Pharmacother. 148:112769. PubMed
  24. Chen K, et al. 2022. Adv Sci (Weinh). 9:e2104106. PubMed
  25. Mise Y, et al. 2022. Carcinogenesis. 43:647. PubMed
  26. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
  27. DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed
  28. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
  29. Zaman R, et al. 2021. Immunity. :. PubMed
  30. Sanmarco LM, et al. 2022. Nature. 611:801. PubMed
  31. Pagni RL, et al. 2022. Front Immunol. 13:1005937. PubMed
  32. Aso K, et al. 2023. Nat Commun. 14:984. PubMed
  33. Bautista CA, et al. 2023. Front Physiol. 14:1122348. PubMed
  34. He Y, et al. 2023. Biomolecules. 13:. PubMed
  35. Lin CH, et al. 2023. iScience. 26:106904. PubMed
  36. Jones NM, et al. 2019. BMC Cancer. 1.127777778. PubMed
  37. John S, et al. 2020. Sci Rep. 10:11377. PubMed
  38. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  39. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  40. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  41. Keeter WC, et al. 2022. Eur Heart J Open. 2:oeac028. PubMed
  42. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  43. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  44. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  45. Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed
  46. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  47. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  48. You Y, et al. 2016. Sci Rep. 6:20630. PubMed
  49. Dong L, et al. 2021. Cancer Cell. . PubMed
  50. Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed
  51. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  52. Shani O, et al. 2020. Cancer Res. 80:5317. PubMed
  53. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  54. McAlpine CS, et al. 2019. Nature. 566:383. PubMed
  55. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  56. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  57. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
  58. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  59. Buzas K, et al. 2011. Cytokine. 55:429. PubMed
  60. Rybalko V, et al. 2015. PLoS One. 10: 0145550. PubMed
  61. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  62. Iwata H, et al. 2016. Nat Commun. 7:12849. PubMed
  63. Uroda T, et al. 2020. Nat Protoc. 15:2107. PubMed
  64. Schlegel M, et al. 2018. J Clin Invest. 128:4711. PubMed
  65. Al-Sharea A, et al. 2017. Cell Cycle. 10.1016/j.atherosclerosis.2017.08.010. PubMed
  66. Bhattacharya P, et al. 2022. PLoS Negl Trop Dis. 16:e0010224. PubMed
  67. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  68. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  69. Moreau GB, et al. 2020. Am J Trop Med Hyg. 103:1215. PubMed
  70. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  71. Mirakaj V, et al. 2014. J Exp Med. 211:1037. PubMed
  72. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  73. Shin SH, et al. 2022. Front Cell Neurosci. 16:844480. PubMed
  74. Xiao P, et al. 2019. J Exp Med. 216:337. PubMed
  75. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  76. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  77. Naler LB, et al. 2022. Commun Biol. 5:102. PubMed
  78. Eichin D, et al. 2015. PLoS One. 10: 0134721. PubMed
  79. Körner A, et al. 2019. Nat Commun. 10:633. PubMed
  80. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  81. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  82. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  83. Körner A, et al. 2019. Proc Natl Acad Sci U S A. 116:20623. PubMed
  84. Kulkarni U, et al. 2019. Mucosal Immunol. 12:545. PubMed
  85. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  86. Liu T, et al. 2022. Front Immunol. 13:901349. PubMed
  87. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  88. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  89. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  90. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  91. Han D, et al. 2021. J Immunol Res. 6688053:2021. PubMed
  92. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  93. Cao Y, et al. 2015. J Biol Chem. 290: 23050-23063. PubMed
  94. Körner A,et al. 2017. Cell Death Differ.. 10.1038/cdd.2017.177. PubMed
  95. He J, et al. 2019. J Clin Invest. 130. PubMed
  96. Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
  97. Monteran L, et al. 2022. Nat Commun. 13:5797. PubMed
  98. Xu Y, et al. 2016. Arthritis Rheum. 67: 225-237. PubMed
  99. Ma H, Wan S, Xia C 2016. J Leukoc Biol. 99: 1121 - 1129. PubMed
  100. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  101. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  102. Smith CA, et al. 2019. JCI Insight. 4. PubMed
  103. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  104. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  105. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  106. Poczobutt JM, et al. 2021. Am J Respir Cell Mol Biol. 64:629. PubMed
  107. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  108. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  109. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  110. Tan X, et al. 2015. Sci Rep. 5: 15702. PubMed
  111. Lee S, et al. 2011. J Immunol. 186:6972. PubMed
  112. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  113. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  114. Garcia-Bonilla L, et al. 2014. J Immunol. 193:2531. PubMed
  115. Alvarez-Breckenridge C, et al. 2012. J Virol. 86:4566. PubMed
  116. Hightower RM, et al. 2019. Mol Ther. 28:189. PubMed
  117. Zhao T, et al. 2022. JCI Insight. 7:. PubMed
  118. Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed
  119. Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed
  120. Hou PP, et al. 2020. Molecular Cell. 78(6):1192-1206.e10. PubMed
  121. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
  122. Moore J, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 906-917. PubMed
  123. León-Cabrera S, et al. 2014. Int J Biol Sci. 9 948 . PubMed
  124. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  125. Chiang N, et al. 2019. J Clin Invest. 129:5294. PubMed
  126. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  127. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  128. Liu Y, et al. 2015. BioMed Res Int. 2015 563425. PubMed
  129. Kwak T, et al. 2020. Cell Reports. 33(13):108571. PubMed
  130. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  131. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  132. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  133. Hobbs BE, et al. 2021. Pathogens. 10:. PubMed
  134. Roediger B et al. 2018. Cell. 175(2):530-543 . PubMed
  135. Meng Michelle Xu et al. 2017. Immunity. 47(2):363-373 . PubMed
  136. Caballero S, et al. 2017. Invest Ophthalmol Vis Sci. 58:5164. PubMed
  137. Donlan AN, et al. 2021. JCI Insight. 6: . PubMed
  138. Tyagi A, et al. 2021. Nat Commun. 12:474. PubMed
  139. Tang S, et al. 2022. Virol J. 19:32. PubMed
  140. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  141. Knuplez E, et al. 2021. British Journal of Pharmacology. 178(5):1234-1248. PubMed
  142. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  143. Nguyen HX et al. 2017. J Neurosci. 37(38):9269-9287 . PubMed
  144. Asahara M, et al. 2022. PLoS One. 17:e0276135. PubMed
  145. Dye BR, et al. 2020. Biomaterials. 234:119757. PubMed
  146. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  147. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  148. Tummala G, et al. 2021. Invest Ophthalmol Vis Sci. 62:41. PubMed
  149. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  150. Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed
  151. Rahtes A, et al. 2020. Front Immunol. 1.484722222. PubMed
  152. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  153. Ngkelo A, et al. 2016. J Exp Med. 213: 1353 - 1374. PubMed
  154. Garcia-Bonilla L, et al. 2015. J Neurosci. 35: 14783 - 14793. PubMed
  155. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  156. Jones GS, et al. 2020. mSphere. 5:. PubMed
  157. Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed
  158. Hegde V, et al. 2015. J Immunol. 194:5211. PubMed
  159. Aguado B, et al. 2015. Sci Rep. 5: 17566. PubMed
  160. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  161. Reinke S, et al. 2020. Cell Reports. 30(8):2501-2511. PubMed
  162. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  163. Abe H, et al. 2019. Nat Commun. 10:2824. PubMed
  164. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  165. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
  166. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
RRID
AB_1186134 (BioLegend Cat. No. 128005)
AB_1186134 (BioLegend Cat. No. 128006)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Other Formats

View All Ly-6C Reagents Request Custom Conjugation
Description Clone Applications
Pacific Blue™ anti-mouse Ly-6C HK1.4 FC
APC anti-mouse Ly-6C HK1.4 FC
Purified anti-mouse Ly-6C HK1.4 FC,IHC-F
Biotin anti-mouse Ly-6C HK1.4 FC
FITC anti-mouse Ly-6C HK1.4 FC
Alexa Fluor® 647 anti-mouse Ly-6C HK1.4 FC
PE anti-mouse Ly-6C HK1.4 FC
PerCP/Cyanine5.5 anti-mouse Ly-6C HK1.4 FC
PE/Cyanine7 anti-mouse Ly-6C HK1.4 FC
Alexa Fluor® 488 anti-mouse Ly-6C HK1.4 FC
Alexa Fluor® 700 anti-mouse Ly-6C HK1.4 FC
APC/Cyanine7 anti-mouse Ly-6C HK1.4 FC
PerCP anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 570™ anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 421™ anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 510™ anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 605™ anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 711™ anti-mouse Ly-6C HK1.4 FC
Purified anti-mouse Ly-6C (Maxpar® Ready) HK1.4 FC,CyTOF®
Brilliant Violet 785™ anti-mouse Ly-6C HK1.4 FC
PE/Dazzle™ 594 anti-mouse Ly-6C HK1.4 FC
APC/Fire™ 750 anti-mouse Ly-6C HK1.4 FC
TotalSeq™-A0013 anti-mouse Ly-6C HK1.4 PG
Brilliant Violet 650™ anti-mouse Ly-6C HK1.4 FC
TotalSeq™-C0013 anti-mouse Ly-6C HK1.4 PG
TotalSeq™-B0013 anti-mouse Ly-6C HK1.4 PG
APC/Fire™ 810 anti-mouse Ly-6C Antibody HK1.4 FC
Spark UV™ 387 anti-mouse Ly-6C HK1.4 FC
PE/Fire™ 810 anti-mouse Ly-6C HK1.4 FC
Spark Blue™ 550 anti-mouse Ly-6C (Flexi-Fluor™) HK1.4 FC
Spark Blue™ 574 anti-mouse Ly-6C (Flexi-Fluor™) HK1.4 FC
PE/Cyanine5 anti-mouse Ly-6C HK1.4 FC
Go To Top Version: 2    Revision Date: 12-09-2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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