- Clone
- M-T271 (See other available formats)
- Workshop
- V 5T CD27.03
- Other Names
- S152, T14, TNFRSF7
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
260404 | 100 tests | 423€ |
CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.
Product DetailsProduct Details
- Reactivity
- Human
- Reported Reactivity
- Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human T cells from a T-ALL patient.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 200 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections1, immunofluorescent staining2, and ELISA3.
- Application References
-
- Ma S, et al. 2011. J. Virol. 85:165. (IHC)
- Manzo A, et al. 2008. Arthritis Rheum. 11:3377. (IF)
- Kato K, et al. 2007. Exp. Hematol. 35:434. (ELISA)
- Disclaimer
-
GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Antigen Details
- Structure
- TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
- Distribution
-
Medullary thymocytes, T and B cell subsets, NK cells
- Interaction
- CD27 binds to CD70
- Ligand/Receptor
- CD70
- Cell Type
- B cells, NK cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Hintzen R, et al. 1994. Immunol. Today 15:307.
4. Agematsu K, et al. 1995. J. Immunol. 154:3627. - Gene ID
- 939 View all products for this Gene ID
- UniProt
- View information about CD27 on UniProt.org
Related FAQs
Other Formats
View All CD27 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD27
Human peripheral blood lymphocytes were stained with purifie... -
FITC anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
PE anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... Human peripheral blood was stained with CD27 (clone M-T271) ... -
PerCP/Cyanine5.5 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
APC anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
PE/Cyanine7 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Pacific Blue™ anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Alexa Fluor® 700 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Brilliant Violet 421™ anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Brilliant Violet 510™ anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
PE/Dazzle™ 594 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
APC/Cyanine7 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Biotin anti-human CD27
Human peripheral blood lymphocytes were stained with biotiny... -
APC/Fire™ 750 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Brilliant Violet 711™ anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
PerCP anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Alexa Fluor® 647 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
KIRAVIA Blue 520™ anti-human CD27
Human Peripheral blood lymphocytes were stained with CD3 APC... -
PE/Cyanine5 anti-human CD27
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Cyanine7 anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
APC anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
PerCP/Cyanine5.5 anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
PE anti-human CD27
Typical result from human peripheral blood lymphocytes stain... -
FITC anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
Brilliant Violet 650™ anti-human CD27
Human peripheral blood lymphocytes were stained with anti-hu... -
Pacific Blue™ anti-human CD27
Typical result from human peripheral lymphocytes stained eit... -
Spark Violet™ 423 anti-human CD27
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE/Cyanine7 anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
Spark Red™ 718 anti-human CD27 (Flexi-Fluor™)
-
GMP PerCP/Cyanine5.5 anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
GMP APC anti-human CD27
Typical results from human peripheral blood lymphocytes stai...
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