GMP Recombinant Human Noggin (carrier-free)

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Other Names
Nog, SYNS1A, SYNS1, SYM1
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GMP_RECOM_Human_Noggin_CF_082823
Recombinant human Noggin inhibits alkaline phosphatase production induced by recombinant human BMP-4 in the chondrogenic cell line ATDC5. The ED50 for this effect is 0.04 – 0.20 µg/mL.
  • GMP_RECOM_Human_Noggin_CF_082823
    Recombinant human Noggin inhibits alkaline phosphatase production induced by recombinant human BMP-4 in the chondrogenic cell line ATDC5. The ED50 for this effect is 0.04 – 0.20 µg/mL.
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795908 25 µg 436€
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795910 100 µg 1452€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

Noggin is an embryonic inducer that can sequester TGF-β cytokines of the BMP family with extremely high affinity. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Noggin also has been shown to modulate the activities of other BMPs including BMP-2, -7, -13, and -14. It is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle, and skin. During culture of human embryonic stem cells or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons. Noggin also appears to maintain adult stem cell populations in vivo. For example, it maintains neural stem cells within the hippocampus. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human Noggin, amino acid (Gln28-Cys232) (Accession: # Q13253) was expressed in E. coli.
Molecular Mass
The 206 amino acid recombinant protein has a predicted molecular mass of approximately 23.17 kD. The DTT-reduced and non-reduced protein migrates at approximately 28 and 70 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Gln.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.1 µm filtered protein solution is in 10 mM citric acid pH 3.0
Endotoxin Level
Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration
25 µg and 100 µg sizes are bottled at 0.5 mg/mL
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Recombinant human Noggin inhibits alkaline phosphatase production induced by recombinant human BMP-4 in the chondrogenic cell line ATDC5. The ED50 for this effect is 0.04 – 0.20 µg/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Disclaimer

GMP Recombinant Proteins. BioLegend GMP recombinant proteins are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

BioLegend GMP recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12.

Antigen Details

Structure
Dimer
Ligand/Receptor
BMP-2, BMP-4, BMP-5, BMP-6, BMP-7, BMP-13, and BMP-14
Bioactivity
Human Noggin inhibits the production of alkaline phosphatase induced by human BMP-4 in ATDC5 cells.
Cell Sources
Preadiposites, skeletal muscle, lung, neuron, skin, adipose tissue, osteoblasts.
Cell Targets
Mesenchymal stem cells, embryonic stem cells
Antigen References
  1. Valenzuela DM, et al. 1995. J. Neurosci. 15:6077.
  2. McMahon JA, et al. 1998. Genes Dev. 12:1438.
  3. Yuasa S, et al. 2005. Nat. Biotechnol. 23:607.
  4. Gong Y, et al. 1999. Nat. Genet. 21:302.
  5. Chiba S, et al. 2008. Stem Cells. 26:2810.
  6. Bonaguidi MA, et al. 2008. J. Neurosci. 28:9194.
  7. Hu X, et al. 2012. J Dent Res. 91:394.
  8. Blázquez-Medela AM, et al. 2019. Mol Met. 25: 50.
  9. Blázquez-Medela AM, et al. 2019. Obes Rev. 20:648.
Regulation
Regulates mainly the function of BMP-2, BMP-4, and BMP-7. Controls the development of bone, muscle, and nerve tissue among others. Modulate adipose tissue and obesity in vivo.
Gene ID
9241 View all products for this Gene ID
UniProt
View information about Noggin on UniProt.org
Go To Top Version: 2    Revision Date: 12-04-2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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