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GMP Recombinant Human PDGF-BB (carrier-free)

Pricing & Availability
Other Names
Simian sarcoma viral oncogene homolog, v-SIS, SSV, SIS, c-sis, PDGF2
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GMP_RECOM_Human_PDGF-BB_Lyophilized_072723
GMP Recombinant human PDGF-BB induces the proliferation of NIH/3T3 cells in a dose-dependent manner. The ED50 for this effect is 1 - 5 ng/mL.
  • GMP_RECOM_Human_PDGF-BB_Lyophilized_072723
    GMP Recombinant human PDGF-BB induces the proliferation of NIH/3T3 cells in a dose-dependent manner. The ED50 for this effect is 1 - 5 ng/mL.
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577325 100 µg 1260€
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Description

PDGF-BB is a mitogen initially identified as simian sarcoma viral oncogene homolog. It belongs to the PDGF family which includes five members, PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC, and PDGF-DD. PDGF-AA, AB, and BB dimers are processed intracellularly and secreted as active dimers that readily activate PDGF receptors (PDGFRs). PDGF-CC and DD are secreted as full-length, latent dimers, and the proteolytic removal of a CUB domain is required for the growth factor domains of PDGF-CC or DD to activate the PDGF receptors. PDGF-BB exerts its biological functions through the activation of dimeric receptors made up of two structurally similar proteintyrosine kinase receptor subunits (αα-, αβ-, or ββ-PDGFR). PDGF has been characterized as a chemoattractant for vascular smooth muscle cells from both sheep and humans and also for canine tracheal myocytes. In ex vivo cutaneous remodeling studies it was revealed that PDGF-BB is a mitogen and chemoattractant for dermal fibroblast. Abnormalities of PDGFR/PDGF are thought to contribute to a number of human diseases including malignancy. Autocrine signaling as a consequence of PDGF-B overexpression is clearly implicated in the pathogenesis of dermatofibrosarcoma protruberans (DFSP).

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human PDGF-BB amino acid (Ser82-Thr190) (Accession: # NM_033016), was expressed in E.coli.
Molecular Mass
The 109 amino acid recombinant protein has a predicted molecular mass of approximately 12.2 kD. The DTT-reduced and non-reduced protein migrates at approximately 13 and 37 kD by SDS-PAGE respectively. The predicted N-terminal amino acid is Ser.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
Protein was lyophilized from 0.2 μm filtered solution contain 0.1% TFA, 10% Acetonitrile.
Endotoxin Level
Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration
100 μg size is lyophilized
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile 4 mM HCL. Before reconstitution, make sure sterile 4 mM HCL and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (sterile 4 mM HCL) to the vial, to obtain the recommended stock concentration 250 μg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all of the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes, and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 μg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/ thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months.
Activity
GMP recombinant human PDGF-BB induces the proliferation of NIH/3T3 cells in a dose-dependent manner. The ED50 for this effect is 1 - 5 ng/mL.
Application

Bioassay
Application Notes

Our liophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Disclaimer

GMP Recombinant Proteins. BioLegend GMP recombinant proteins are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

BioLegend GMP recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12.

Antigen Details

Structure
Homodimer
Distribution

PDGF-BB is mainly expressed in vascular endothelial cells, megakaryocytes, and neurons.

Function
PDGF-BB regulates diverse cellular processes, including cell proliferation, transformation, migration, survival, and apoptosis of mesenchymal cells, in development and during pathogenesis.
Interaction
Mesenchymal-derived cells
Ligand/Receptor
αα-PRGFR, αβ-PDGFR, and ββ-PDGFR
Bioactivity
Recombinant human PDGF-BB induces the proliferation of NIH/3T3 cells in a dose-dependent manner.
Antigen References
  1. Carlin SM, et al. 2003. Am J Physiol Lung Cell MoPhysiol. 284:L1020.
  2. Ustach CV, et al. 2005. Mol Cell Biol. 25:6279.
  3. Jones AV and Cross NC. 2004. Cell Mol Life Sci. 61:2912.
  4. Andrae J, et al. 2008. Genes Dev. 22:1276.
  5. Banerjee P, et al. 2011. J. Biol Chem. 23:33580
Gene ID
5159 View all products for this Gene ID
UniProt
View information about PDGF-BB on UniProt.org
Go To Top Version: 1    Revision Date: 08-08-2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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