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LEAF™ Purified anti-human PDGF-AA Antibody

Pricing & Availability
Clone
Poly5355 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet-Derived Growth Factor-AA, Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly5355_LEAF_PDGF-AA_Antibody_1_012122
Anti-human PDGF-AA inhibits NIH 3T3 cell proliferation induced by 300 ng/mL recombinant human PDGF-AA (Cat. No. 773702). The ND50 for this effect is 2-10 µg/mL.
  • Poly5355_LEAF_PDGF-AA_Antibody_1_012122
    Anti-human PDGF-AA inhibits NIH 3T3 cell proliferation induced by 300 ng/mL recombinant human PDGF-AA (Cat. No. 773702). The ND50 for this effect is 2-10 µg/mL.
  • Poly5355_LEAF_PDGF-AA_Antibody_2_012122
    IHC staining of purified anti-human PDGF-AA (clone Poly5355) on formalin-fixed paraffin-embedded human breast cancer (A, positive control) and breast (B, negative control) tissues. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissues were incubated with 5 µg/mL of purified anti-human PDGF-AA overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) and HRP anti-goat antibody were used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 μm
  • Poly5355_LEAF_PDGF-AA_Antibody_3_012122
    Total cell lysates (15 µg total protein) from Jurkat (negative control, Lane 1) and Jurkat lysate spiked with various amounts of recombinant human PDGF-AA protein (Cat. No. 773702) at 100 ng (positive control, Lane 2), 50 ng (positive control, Lane 3), and 10 ng (positive control, Lane 4) were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 μg/mL of purified anti-human PDGF-AA overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP anti-goat IgG. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight ladder
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535503 100 µg 352€
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Description

Platelet-derived growth factors (PDGFs) are homo- or heterodimers of four polypeptides, known as A, B, C and D chains. PDGFs usually act primarily in paracrine manner and may be engaged in autocrine loops in tumors. Five PDGF isoforms (AA, BB, AB, CC and DD) have been identified. PDGFs are primarily synthesized, stored, and released by platelets upon activation. PDGF-A and PDGF-B undergo intracellular activation during transport in the exocytic pathway and PDGF-C and PDGF-B are activated by extracellular proteases. Like PDGF-B, intracellular activation of PDGF-A, by removing the N-terminal sequence, is required for PDGF-A to bind to its receptor. PDGF-A can also be expressed by epithelial cells, smooth muscle cells, and neuronal progenitors. PDGF-AA is well known as a potent mitogen for the proliferation of oligodendrocyte progenitor cells (OPCs). PDGF-AA can promote embryonic forebrain neural stem cells into O4-positive oligodendrocytes by activating Erk pathway. PDGF-AA also plays an important role in directing cell migration during embryonic development and postnatal migration response including wound healing. Chemotaxis ability of PDGF-AA has been shown on fibroblasts, smooth muscle cells, monocytes, and granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human PDGF-AA
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
IHC-P, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of NIH 3T3 cell proliferation induced by recombinant human PDGF-AA (Cat. No. 773702) at 300 ng/mL. The ND50 is 2 – 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910467 (BioLegend Cat. No. 535503)

Antigen Details

Structure
Homodimer
Distribution

Platelets produce PDGF-AA. Other cells, including epithelial cells, smooth muscle cells, and neuronal progenitors, can also produce PDGF-AA.

Function
PDGF-A has two isoforms due to alternative splicing which is cell type-specific and is different among organs and tumor cell lines. Expression of PDGF-A can be increased in human uterine smooth muscle cells during the physiological hypertrophy. Expression of PDGF-A can be repressed by glucocorticoid treatment of smooth muscle cells and aging of human fibroblasts.
Interaction
Smooth muscle cells, connective tissue cells, bone and cartilage cells, and oligodendrocyte cells
Ligand/Receptor
PDGFR-α
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Heldin CH and Westermark B. 1999. Physiol Rev. 79:1283-316.
  2. Fredriksson L, et al. 2004. Cytokine Growth Factor Rev. 15:197-204.
  3. Hu JG, et al. 2012. J Mol Neurosci. 46:644-53.
  4. Schneider L, et al. 2010. Cell Physiol Biochem. 25:279-92.
  5. Karlsson C and Paulsson Y. 1994. J Cell Physiol. 158:256-62.
  6. Shure D, et al. 1992. Biochem Biophys Res Commun. 186:1510-4.
Gene ID
5154 View all products for this Gene ID
UniProt
View information about PDGF-AA on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 01-21-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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