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LEAF™ Purified anti-mouse ACE2 Antibody

Pricing & Availability
Clone
Poly5270 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly5270_LEAF_ACE2_Antibody_1_011422
LEAF™ purified anti-mouse ACE2 (clone Poly5270) inhibits the cleavage of fluorogenic substrate Mca-YVADAPK(Dnp)-OH by recombinant mouse ACE2 (Cat. 720104). The IC50 for this effect is 1.5 - 7.5 µg/mL in the presence of 25 ng mouse ACE2 per sample and 10 µM substrate.
  • Poly5270_LEAF_ACE2_Antibody_1_011422
    LEAF™ purified anti-mouse ACE2 (clone Poly5270) inhibits the cleavage of fluorogenic substrate Mca-YVADAPK(Dnp)-OH by recombinant mouse ACE2 (Cat. 720104). The IC50 for this effect is 1.5 - 7.5 µg/mL in the presence of 25 ng mouse ACE2 per sample and 10 µM substrate.
  • Poly5270_LEAF_ACE2_Antibody_2_011422
    Whole cell extracts (15 µg protein) from HeLa cells (negative control), human kidney, mouse kidney, and mouse liver tissue were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. Membranes were probed with 1.0 µg/mL of purified anti-mouse ACE2 (clone Poly5270) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP anti-goat IgG. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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527003 100 µg 352€
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Description

ACE2 is a member of the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases. It catalyzes the cleavage of the decapeptide angiotensin I into angiotensin-(1-9) and angiotensin II (potent vasoconstrictor) into the vasodilator angiotensin-(1-7). ACE2 is a type I membrane protein that functions as a carboxypeptidase. It cleaves between a proline and a single hydrophobic/basic residue from the COOH-terminus of its substrates. The mouse full-length enzyme possesses 805 amino acids and the ECD includes amino acids 1–740. ACE2 is a zinc metalloprotease with considerable homology to angiotensin I-converting enzyme (ACE). Both enzymes contain the typical HEXXH zinc-binding motif, ACE has two catalytic sites and ACE2 has only one, and ACE2 is not inhibited by ACE inhibitors captopril, lisinopril, and enalaprilat. Studies in mice showed that disruption of ACE2 induced a severe cardiac contractility defect and increased angiotensin II levels in heart. Human ACE2 has been identified as the receptor for SARS (severe acute respiratory syndrome)-coronavirus. ACE2 binds to the coronavirus S protein present on the surface of the virion. Studies in SARS-CoV-ACE2 interactions showed that specific amino acids (Lys31 and Lys353) in human ACE2 were critical to virus-receptor binding, and naturally selected viral mutations in S1 (K479N and S487T) enhanced the affinity of S1 for human ACE2. Rat ACE2 is not a receptor for SARS-CoV and mouse ACE2 is a poor receptor. Rat ACE2 has two amino acids that do not favor the binding of coronavirus S protein, one of which is His353 that mouse ACE2 also possesses. Therefore, rat cells are not infected by SARS-CoV and mouse cells are infected inefficiently.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant mouse ACE2
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Block - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by inhibiting the cleavage of 10 µM Mca-YVADAPK(Dnp)-OH substrate by 25 ng mouse ACE2. The IC50 for this effect is 1.5 - 7.5 µg/mL. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910466 (BioLegend Cat. No. 527003)

Antigen Details

Structure
Monomer
Distribution

Vascular endothelial cells of the heart, kidney, brain, and testis

Function
Regulates renal and cardiovascular function
Interaction
Angiotensin I and angiotensin II are substrates for ACE2.
Ligand/Receptor
Receptor for SARS-CoV-2 entry
Cell Type
Monocytes
Biology Area
Angiogenesis, COVID-19, Immunology
Molecular Family
Enzymes and Regulators
Antigen References
  1. Crackower MA, et al. 2002. Nature. 417:822-8.
  2. Vickers C, et al. 2002. J Biol Chem. 277:14838-43.
  3. Tikellis C, et al. 2003. Hypertension. 41:392-7.
  4. Guan Y, et al. 2003. Science. 302:276-8. 
  5. Li F, et al. 2005. Science. 309:1864-8.
  6. Ferrario CM, et al. 2005. Am J Physiol Heart Circ Physiol. 289: H2281-90.
  7. Perlman S & Netland J. 2009. Nat Rev Microbiol. 7:439-50.
  8. Wu K, et al. 2011. J Virol. 85:5331-7.
  9. Ge XY, et al. 2013. Nature. 503:535-8.
  10. Letko M, et al. 2020. Nat Microbiol. 5:562-569.

 

Gene ID
70008 View all products for this Gene ID
UniProt
View information about ACE2 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 01-14-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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