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- Regulatory Status
- RUO
- Other Names
- IL-4, Periostin, IL-5, IL-33, TSLP, RAGE, IL-13, IL-9, CCL11 (Eotaxin)
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Allergic diseases including asthma are mediated by T-helper 2 (Th2)-biased immune responses together with isotype switching of B cells to generate IgE antibodies specific for common environmental allergens. Th2 cytokines such as IL-4, IL-5, IL-9 and IL-13 play a significant role in allergic reactions, which can be further amplified by IL-33 with its ST2 binding and activation. TSLP, produced by epithelial cells in the airways, also drive those Th2 cytokines by stimulating dendritic cells into maturation. Periostin, another molecule secreted by airway epithelial cells, can be upregulated by IL-4 and IL-13. Asthma and allergic inflammation are also induced through RAGE receptor binding of advanced glycation end products. Moreover, MMP-9 of the matrix metalloproteinase family is responsible for extracellular matrix degradation and airway remodeling, with its increase correlated with asthma severity. Similarly, YKL-40, a chitinase-like protein, is significantly elevated in asthmatic patients’ serum, indicating a potential biomarker for the disease. CCL11 (Eotaxin), a CC chemokine, is another crucial player in recruitment and migration of eosinophils from peripheral blood into the lung to perpetuate asthma and allergic inflammation through CCR3 binding, which results in subsequent airway remodeling through profibrogenic activity. Examining the various cytokines and molecules associated with allergies and asthma can lead to improved insights for developing effective treatments in the future.
The LEGENDplex™ Human Allergy/Asthma Panel is a bead-based multiplex assay, utilizing fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 11 human antibodies related to allergy and asthma, including IL-4, Periostin, IL-5, IL-33, TSLP, RAGE, IL-13, YKL-40, MMP-9, IL-9, CCL11 (Eotaxin). This panel assay provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated for use on serum, plasma and cell culture supernatant samples.
Kit Contents
- Kit Contents
-
- Setup Beads: PE Beads
- Setup Beads: Raw Beads
- LEGENDplex™ Human Allergy/Asthma Panel Capture Beads
- LEGENDplex™ Human Allergy/Asthma Panel Detection Abs
- LEGENDplex™ Human Allergy/Asthma Panel Standard
- LEGENDplex™ Lyophilized Standard Reconstitution Buffer
- LEGENDplex™ Matrix C, Lyophilized
- LEGENDplex™ SA-PE
- LEGENDplex™ Assay Buffer
- LEGENDplex™ Wash Buffer, 20X
- Plate Sealers
- Filter Plate
Product Details
- Verified Reactivity
- Human
- Application
-
Multiplex - Panel
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here. - Materials Not Included
-
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- Centrifuge with a swinging bucket adaptor for microtiter plates (if using V-bottom plates)
- 1.1 mL polypropylene micro FACS tubes, in 96-tube rack
- Manual
Antigen Details
- Cell Type
- Epithelial cells
- Biology Area
- Adaptive Immunity
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 3565 View all products for this Gene ID 10631 View all products for this Gene ID 3567 View all products for this Gene ID 90865 View all products for this Gene ID 85480 View all products for this Gene ID 177 View all products for this Gene ID 3596 View all products for this Gene ID 3578 View all products for this Gene ID 6356 View all products for this Gene ID
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
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If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
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Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
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The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
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LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
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Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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