- Regulatory Status
- RUO
- Other Names
- IFN-α2, TSLP, IL-1α, IL-1β, GM-CSF, IL-11, IL-12p40, IL-12p70, IL-15, IL-18, IL-23, IL-27, IL-33
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cytokines, which are soluble extracellular proteins or glycoproteins, are key regulators of cells. They are secreted by immune cells and act on other cells to coordinate immune responses. They are crucial for innate and adaptive host defenses, cell growth, death, differentiation, as well as development and repair processes.
The Human Cytokine Panel 2 V02 is a multiplex bead-based assay panel, suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 human cytokines, including TSLP, IL-1α, IL-1β, GM-CSF, IFN-α2, IL-23, IL-12p40, IL-12p70, IL-15, IL-18, IL-11, IL-27, and IL-33. This panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated for evaluation of expected changes in biological samples.
Kit Contents
- Kit Contents
-
- Setup Beads: PE Beads
- Setup Beads: Raw Beads
- LEGENDplex™ Human Cytokine Panel 2 V02 Panel Premixed Beads
- LEGENDplex™ Human Cytokine Panel 2 V02 Detection Abs
- LEGENDplex™ Human Cytokine Panel 2 V02 Std Cktl, Lyoph.
- SA-PE
- Matrix A, Lyophilized
- Assay Buffer
- Wash Buffer
- Filter Plate
- Plate Sealers
Product Details
- Verified Reactivity
- Human
- Application
-
Multiplex - Panel
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here. - Materials Not Included
-
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent pads or paper towels
- Plate Shaker
- Tabletop Centrifuges
- 1.1 mL polypropylene micro FACS tubes, in 96-tube rack
Antigen Details
- Gene ID
- 85480 View all products for this Gene ID 3552 View all products for this Gene ID 3553 View all products for this Gene ID 1437 View all products for this Gene ID 3439 View all products for this Gene ID 3440 View all products for this Gene ID 3593 View all products for this Gene ID 51561 View all products for this Gene ID 3592 View all products for this Gene ID 3593 View all products for this Gene ID 3600 View all products for this Gene ID 3606 View all products for this Gene ID 3589 View all products for this Gene ID 246778 View all products for this Gene ID 90865 View all products for this Gene ID
Related Pages & Pathways
Pages
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
-
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
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Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
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The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
-
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
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Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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