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- Regulatory Status
- RUO
- Other Names
- pTau217, pTau181, pTau262, pTau (Thr217), pTau (Thr181), pTau (Ser262), Tau, Microtubule-Associated Protein Tau, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1
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- Product Citations
- publications
Tau is a 441-amino acid protein that binds along microtubules to stabilize them. The hyperphosphorylation of Tau reduces its affinity for microtubules, leading to destabilization. Tau in healthy neurons carries multiple phosphate groups. Once highly phosphorylated, Tau protein assembles into higher order structures, including oligomers and neurofibrillary tangles (NFT). Elevated phosphorylation and aggregation of Tau are widely considered pathological hallmarks in Alzheimer’s disease (AD), Parkinson's disease (PD), and other neurodegenerative diseases. Therefore, the measurement of pathological Tau alterations including the level of phosphorylation and aggregation both serve as important biomarker predictions for the onset and progression for Alzheimer's disease.
The LEGENDplex™ Human Phospho-Tau Panel (3-plex) is a bead-based multiplex assay, using fluorescence-encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 3 key phosphorylated Tau targets considered biomarkers in various neurodegenerative diseases including pTau217, pTau181, pTau262. This assay panel provides higher sensitivity and a broader dynamic range than traditional ELISA methods. The panel has been validated for use on human brain lysate, cerebrospinal fluid (CSF), cell culture supernatant, serum, and plasma.
Kit Contents
- Kit Contents
-
- Setup Beads: PE Beads
- Setup Beads: Raw Beads
- LEGENDplex™ Human Phospho-Tau (3-plex) Panel Premixed Beads
- LEGENDplex™ Human Phospho-Tau Panel Detection Antibodies
- LEGENDplex™ Human Phospho-Tau Panel Standard
- LEGENDplex™ Matrix A, Lyophilized
- LEGENDplex™ SA-PE
- LEGENDplex™ Assay Buffer
- LEGENDplex™ Wash Buffer, 20X
- Plate Sealers
- Filter Plate
Product Details
- Verified Reactivity
- Human
- Application
-
Multiplex - Panel
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here. - Materials Not Included
-
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- 1.1 mL polypropylene micro FACS tubes, in 96-tube rack
- Manual
Antigen Details
- Cell Type
- Neurons
- Biology Area
- Neurodegeneration, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microtubules, Tau
- Gene ID
- NA
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
-
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
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Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
-
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
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LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
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Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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