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Pacific Blue™ anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
MEM-188 (See other available formats)
Regulatory Status
RUO
Workshop
VI NK26
Other Names
NCAM, Leu-19, NKH1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
MEM-188_Blue_080709.jpg
Human peripheral blood lymphocytes stained with MEM-188 Pacific Blue™
  • MEM-188_Blue_080709.jpg
    Human peripheral blood lymphocytes stained with MEM-188 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
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304629 100 tests 226€
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Description

CD56 is a single transmembrane glycoprotein also known as N-CAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cow, Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KG-1 human acute myelogenous leukemia cell line
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The CD56 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
For µg size, the suggested use of this reagent for immunofluorescent staining is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, and Western blotting (non-reducing).

Application References
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Product Citations
  1. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  2. Autenrieth S, et al. 2015. Clin Transl Immunology. 4:50. PubMed
  3. Hanaoka N, et al. 2010. J Immunol. 185:5732. PubMed
RRID
AB_2282499 (BioLegend Cat. No. 304629)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein, 175-185 kD
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org
Go To Top Version: 2    Revision Date: 12-16-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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