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Pacific Blue™ anti-human CD86 Antibody

Pricing & Availability
Clone
IT2.2 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD86.8
Other Names
B7-2, B70
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
IT2dot2_PBlue_111407
Human peripheral blood monocytes stained with IT2.2 Pacific Blue™
  • IT2dot2_PBlue_111407
    Human peripheral blood monocytes stained with IT2.2 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
305417 25 µg 100€
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305418 100 µg 212€
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305423 100 tests 226€
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Common Marmoset, Cotton-topped Tamarin, Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD86 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per 106 cells in 100 µl volume.
For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤0.5 µg per 106 cells in 100 µl volume.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, Western blotting3, and blocking of T cell activation2,4,5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).

Application References
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Dieu M. 1998. J. Exp. Med. 188:373. (Block)
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Jeannin P, et al. 1999. J. Immunol. 162:2044. (Block)
  5. Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. (Block)
  6. Geissmann F, et al. 2001. Blood 97:1241. (IHC)
Product Citations
  1. Feuerer N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  2. Groen R, et al. 2015. J Leukoc Biol. 98: 623 - 630. PubMed
  3. Thwe PM et al. 2017. Cell metabolism. 26(3):558-567 . PubMed
  4. Gustafsson R, et al. 2013. PLoS One. 8:e58122. PubMed
  5. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  6. Beljanski V, et al. 2015. J Virol. 89: 10612 - 10624. PubMed
  7. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  8. Mansour H, et al. 2010. J Infect Dis. 202:1671. PubMed
  9. Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
  10. Soema P, et al. 2015. Eur J Pharm Biopharm. 94: 427-435. PubMed
  11. Tavano B, et al. 2013. J Immunol. 190:2622. PubMed
  12. Chao Y, et al. 2014. J Leukoc Biol. 96:1037. PubMed
  13. Liu W, et al. 2012. Vaccine. 30:1659. PubMed
  14. Teirlinck A, et al. 2015. Infect Immun . 83: 3732-3739. PubMed
  15. Rusconi M, et al. 2017. Sci Rep. 5.745138889. PubMed
  16. Brummelman J, et al. 2015. Infect Immun . 83:227. PubMed
  17. Tan EE, et al. 2020. J Clin Invest. 130:5817. PubMed
RRID
AB_493662 (BioLegend Cat. No. 305417)
AB_493662 (BioLegend Cat. No. 305418)
AB_493662 (BioLegend Cat. No. 305423)

Antigen Details

Structure
Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution

Monocytes/macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hathcock K, et al. 1996. Adv. Immunol. 62:131.
2. June C, et al. 1994. Immunol. Today 15:321.

Gene ID
942 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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