Pacific Blue™ anti-mouse CD31 (PECAM-1) Antibody, CD31, W18222B

Pricing & Availability

Clone: W18222B (See other available formats)
Regulatory Status: RUO
Other Names: PECAM-1, EndoCAM
Isotype: Rat IgG2b, κ
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Product Citations: publications

W18222B_PB_CD31_Antibody_122923 — C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (clone 17A2) FITC and anti-mouse CD31 (clone W18222B) Pacific Blue™ (left) or rat IgG2b, κ Pacific Blue™ isotype control (right).

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160215

25 µg

96€

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160216

100 µg

192€

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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α_v β₃ integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD31 transfected cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
RRID: AB_3106007 (BioLegend Cat. No. 160215)
AB_3106007 (BioLegend Cat. No. 160216)

Antigen Details

Structure: Ig superfamily, 130-140 kD
Distribution: Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
Function: Adhesion
Ligand/Receptor: CD38, α_V/β₃ integrin
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.

DeLisser HM, et al. 1994. Immunol Today. 15:490-5.

Newman PJ, et al. 1990. Science. 247:1219-22.
Gene ID: 18613 View all products for this Gene ID
UniProt: View information about CD31 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Other Formats

View All CD31 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse CD31 (PECAM-1)	W18222B	FC
Purified anti-mouse CD31 (PECAM-1)	W18222B	FC,IHC-F
Alexa Fluor® 488 anti-mouse CD31 (PECAM-1) Antibody	W18222B	FC
APC anti-mouse CD31 (PECAM-1)	W18222B	FC
PE/Cyanine7 anti-mouse CD31 (PECAM-1)	W18222B	FC
FITC anti-mouse CD31 (PECAM-1)	W18222B	FC
PerCP/Cyanine5.5 anti-mouse CD31 (PECAM-1)	W18222B	FC
Pacific Blue™ anti-mouse CD31 (PECAM-1)	W18222B	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12-29-2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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