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Pacific Blue™ anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Antibody

Pricing & Availability
Clone
GL7 (See other available formats)
Regulatory Status
RUO
Other Names
Ly77, T and B cell activation marker
Isotype
Rat IgM, κ
Ave. Rating
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Product Citations
publications
GL7_PB_GL7_Antibody_FC_1_032514
BALB/c mouse bone marrow cells were stained with anti-mouse IgD APC and GL7 (clone GL7) Pacific Blue™ (top) or rat IgM, κ Pacific Blue™ isotype control (bottom).
  • GL7_PB_GL7_Antibody_FC_1_032514
    BALB/c mouse bone marrow cells were stained with anti-mouse IgD APC and GL7 (clone GL7) Pacific Blue™ (top) or rat IgM, κ Pacific Blue™ isotype control (bottom).
  • GL7_PB_GL7_Antibody_FC_2_032514
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
144613 25 µg 90€
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144614 100 µg 231€
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Description

The GL7 antigen, also known as Ly77, is a 35 kD protein.  The GL7 antigen has an epitope containing non-sulfated α2-6-sialyl-LacNAc recognized by the GL7 antibody. The GL7 antigen is expressed by pre-B and immature B cells, activated T and B cells, and about 20% of TCR-bright thymocytes.  It is upregulated on mouse splenocytes following activation.  It may play a role in regulation or adhesion.  GL7 high-expressing B cells show higher antibody production and antigen presenting capacity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS activated DBA/J mouse B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References
  1. Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
  2. Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
  3. Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
  4. Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
  5. Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
Product Citations
  1. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  2. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
  3. Hu Q, et al. 2022. Cell Rep. 40:111035. PubMed
  4. Lin J, et al. 2022. Adv Sci (Weinh). 9:e2202633. PubMed
  5. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  6. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  7. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  8. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  9. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  10. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  11. Huang L, et al. 2017. PLoS Biol.. 10.1371/journal.pbio.2001750. PubMed
  12. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  13. Kato Y, et al. 2020. Immunity. 53(3):548-563. PubMed
  14. Duan L, et al. 2021. Immunity. 54:2256. PubMed
  15. Doron I, et al. 2021. Cell. 184(4):1017-1031.e14. PubMed
  16. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  17. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
  18. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  19. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  20. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  21. Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
  22. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  23. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  24. Zhou X, et al. 2021. Cell Reports. 34(1):108601. PubMed
  25. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  26. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  27. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  28. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  29. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  30. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  31. Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed
RRID
AB_2563291 (BioLegend Cat. No. 144613)
AB_2563291 (BioLegend Cat. No. 144614)

Antigen Details

Structure
35 kD
Distribution

Germinal center B cells, activated B and T cells

Function
Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
Ligand/Receptor
Neu5Ac-recognizing lectins
Cell Type
B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.

Gene ID
NA
UniProt
View information about GL7 on UniProt.org
Go To Top Version: 1    Revision Date: 03-26-2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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