Pacific Blue anti-mouse Lineage Cocktail with Isotype Ctrl

Pricing & Availability

Clone: 17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70;
Regulatory Status: RUO
Other Names: Lin-1
Isotype: Rat IgG2a/Rat IgG2b
Ave. Rating: Submit a Review
Product Citations: publications

Lineage_Cocktail_PB_1_112811 — C57BL/6 bone marrow cells were stained with CD117 FITC and Pacific Blue™ lineage cocktail (top plot) or Pacific Blue™ isotype control cocktail (bottom plot).

Compare all formats

Cat #

Size

Price

Quantity Check Availability

Save

133305

25 tests

235€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

133306

100 tests

469€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Pacific Blue™ Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 17A2; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. Pacific Blue™ Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.

Materials provided:
1. Pacific Blue™ anti-mCD3/ Pacific Blue™ anti-mLy-6G(Ly-6C)/ Pacific Blue™ anti-mCD11b/ Pacific Blue™ anti-mCD45R(B220)/ Pacific Blue™ anti-mTer-119
2. Pacific Blue™ Rat IgG2b/Rat IgG2a isotype controls

Materials not provided:
Cell Staining Buffer (Cat. No. 420201)

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Host Species: Rat
Formulation: This two-reagent set contains Pacific Blue™ conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
Preparation: The antibodies are purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Storage & Handling: The antibody solutions should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_11126168 (BioLegend Cat. No. 133305)
AB_11126978 (BioLegend Cat. No. 133306)

Antigen Details

Distribution: T cells, B cells, monocytes/macrophages, granulocytes, NK cells, and erythrocytes
Cell Type: B cells, Erythrocytes, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area: Immunology
Antigen References: 1. Morrison SJ, et al. 1997. Development 124:1929.
2. Okada S, et al. 1992. Blood 80:3044.
3. Spangrude GJ, et al. 1988. Science 241:58.
4. Spangrude GJ, et al. 1990. Exp. Hematol. 18:920.
5. Tan SL, et al. 2006. J. Immunol. 176:2872.
Gene ID: 104231 View all products for this Gene ID
UniProt: View information about Lineage on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All Lineage Reagents Request Custom Conjugation

Description	Clone	Applications
Pacific Blue™ anti-mouse Lineage Cocktail with Isotype Ctrl	17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70	FC
Pacific Blue™ anti-mouse Lineage Cocktail	17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70	FC
Brilliant Violet 421™ anti-mouse Lineage Cocktail	17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70	FC
Alexa Fluor® 700 anti-mouse Lineage Cocktail with Isotype Ctrl	17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70	FC

Certificate of Analysis

Customers Also Purchased

7-AAD Viability Staining Solution

Alexa Fluor® 700 anti-mouse Ly-6A/E (Sca-1)

Clone: D7

APC/Cyanine7 anti-mouse CD117 (c-kit)

Clone: 2B8

APC/Fire™ 750 anti-mouse CD117 (c-Kit)

Clone: 2B8

Go To Top Version: 3 Revision Date: 07-09-2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.