PE anti-human Basophils 2D7 Antibody anti-Basophil

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Clone: Basophil/2D7 (See other available formats)
Regulatory Status: RUO
Isotype: Mouse IgG1, κ
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Product Citations: publications

Basophil2D7_PE_1_022212 — Human peripheral blood mononuclear cells were stained with CCR3 APC, fixed, permeabilized and then stained with Basophil/2D7 PE (top) or mouse IgG1, κ PE isotype control (bottom). Data shown was gated on the lymphoid cell population.

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346206

100 tests

278€

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Description

The Basophil/2D7 mAb specifically recognizes the human basophil population. The antigen that this antibody binds is present in cell granules but its identity is still unknown.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Lysates of purified basophils
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications for the relevant formats include: immunofluorescence staining⁷, and immunohistochemistry of acetone-fixed frozen sections¹ and formalin-fixed paraffin-embedded sections^1-6.
RRID: AB_11125171 (BioLegend Cat. No. 346206)

Antigen Details

Distribution: Basophils.
Cell Type: Basophils
Biology Area: Immunology, Innate Immunity
Gene ID: NA
UniProt: View information about Basophil on UniProt.org

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Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All Basophil Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human Basophils (2D7)	Basophil/2D7	ICFC,ICC,IHC-P,IHC-F
Alexa Fluor® 647 anti-human Basophils (2D7)	Basophil/2D7	ICFC
PE anti-human Basophils (2D7)	Basophil/2D7	ICFC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 05-31-2013

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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