Loading...

PE anti-human BLNK Phospho (Tyr96) Antibody

Pricing & Availability
Clone
A19008A (See other available formats)
Regulatory Status
RUO
Other Names
B Cell Linker, SLP-65, BLNK-S, BASH
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
A19008A_PE_BLNK_Antibody_031422
Human peripheral blood lymphocytes were treated with (left) or without (right) IgM (12 µg/mL) for 3 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained intracellularly with anti-human CD20 APC and anti-human BLNK Phospho (Tyr96) (clone A19008A) PE.
  • A19008A_PE_BLNK_Antibody_031422
    Human peripheral blood lymphocytes were treated with (left) or without (right) IgM (12 µg/mL) for 3 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained intracellularly with anti-human CD20 APC and anti-human BLNK Phospho (Tyr96) (clone A19008A) PE.
See PE spectral data
Cat # Size Price Quantity Check Availability Save
382203 25 tests 128€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
382204 100 tests 296€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

B cell linker protein (BLNK), an adapter protein molecule, which plays important roles in B cell antigen receptor (BCR) engagement and B cell activation. Cross-linking of the BCR stimulates various downstream signaling pathways involved in cell proliferation and apoptosis. Intracellular protein tyrosine kinases such as Syk and Lyn have been implicated in this BCR signal transduction and are thought to play an important role in B cell development. The two forms of BLNK, p68 and p70, arise from alternate splicing of the human BLNK gene transcript. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for effector proteins GRB2, Vav, Nck and PLCγ2, which in turn leads to the activation of downstream signaling pathways.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human BLNK (phosphorylated at Tyr 96)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2924580 (BioLegend Cat. No. 382203)
AB_2924580 (BioLegend Cat. No. 382204)

Antigen Details

Structure
Human BLNK is 456 amino acids (aa) in length and it contains a Pro­rich region (aa 98­260) and one SH2 domain (aa 346­453).
Distribution

Cytosol, plasma-membrane associated, B cells

Function
Links B cell receptor engagement with downstream signaling events
Interaction
PLCG1, PLCG2, VAV1, NCK1, VAV3, GRB2, FYN, LYN, SYK
Molecular Family
Phospho-Proteins
Antigen References
  1. Fu C, et al. 1998. Immunity 9:93-103.
  2. Ishiai M, et al. 1999. Immunity 10:117-25.
  3. Baba Y, et al. 2001. Proc. Natl. Acad. Sci. USA 98:2582-6.
  4. Tsuji S, et al. 2001. J. Exp. Med. 194:529-39.
  5. Chiu CW, et al. 2002. EMBO. J. 21:6461-72.
  6. Taguchi T, et al. 2004. Immunology. 122:575-82.
Gene ID
29760 View all products for this Gene ID
UniProt
View information about BLNK Phospho Tyr96 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03-14-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account