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PE anti-human Siglec-8 Antibody

Pricing & Availability
Clone
7C9 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid-binding Ig-like lectin 8 (Siglec-8), Siglec8L, Sialoadhesin family member 2 (SAF2)
Isotype
Mouse IgG1, κ
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Product Citations
publications
7C9_PE_1_061112
Human peripheral blood leukocytes were stained with CCR3 APC and Siglec-8 (clone 7C9) PE (top) or mouse IgG1, κ PE isotype control (bottom). Data shown was gated on the granulocyte cell population.
  • 7C9_PE_1_061112
    Human peripheral blood leukocytes were stained with CCR3 APC and Siglec-8 (clone 7C9) PE (top) or mouse IgG1, κ PE isotype control (bottom). Data shown was gated on the granulocyte cell population.
  • 7C9_PE_2_061112
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347103 25 tests 123€
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347104 100 tests 288€
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Description

Siglec-8 is a lectin specific for 6'-sulfo-sLex and a member of the Ig-superfamily. It is expressed almost exclusively in eosinophils; however, basophils and mast cells can express it to a lower degree. Siglec-8 is a 54 kD transmembranal protein; the extracellular domain has one V-set Ig-like domain and two C2-set domains. The cytoplasmic domain has two immunoreceptor tyrosine-based inhibitor motifs (ITIM) that recruit SH2-family phosphatases after tyrosine phosphorylation. There are reports that siglec-8 inhibits the release of histamine and prostaglandin D2 mediated by the IgEFcR. This molelcule is also involved in the induction of apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Siglec-8 fused to human IgG Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
  1. Floyd H, et al. 2000. J. Biol. Chem. 275:861.
  2. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations
  1. Youngblood BA, et al. 2020. Cells. 10:. PubMed
  2. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
  3. Wong T, Jelinek D 2013. J Immunol Methods. 387:130. PubMed
  4. Gadkari M, et al. 2018. Sci Data. 5:180275. PubMed
  5. Duan S, et al. 2021. J Immunol. 206:2290. PubMed
  6. Lin J, et al. 2017. Sci Rep. 7:41722. PubMed
  7. Hernandez JD, et al. 2020. Sci Rep. 10:13210. PubMed
  8. Serra EG, et al. 2020. Nat Commun. 11:995. PubMed
  9. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  10. Wright A, et al. 2016. J Immunol. 196: 4877 - 4884. PubMed
  11. Fan Z, et al. 2020. STAR Protoc. 1:100012. PubMed
  12. Legrand F, et al. 2019. J Allergy Clin Immunol. 143:2227. PubMed
  13. Motwani MP, et al. 2018. JCI Insight. 3:e94463. PubMed
  14. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  15. Jenner W, et al. 2014. PLoS One. 9:89375. PubMed
  16. Lippeveld M, et al. 2020. Cytometry A. 97:308. PubMed
RRID
AB_2270438 (BioLegend Cat. No. 347103)
AB_2270438 (BioLegend Cat. No. 347104)

Antigen Details

Structure
A lectin of 54 kD, belonging to the Ig-superfamily; has one V-set Ig-like domain and two C2-set domains; has a single transmembrane domain, and two immunoreceptor tyrosine-based inhibitor motifs (ITIM) in the cytoplasmic domain
Distribution

Eosinophils, basophils, mast cells

Function
Cell adhesion, signal transduction
Interaction
Phosphatases containing a SH2 domain
Bioactivity
Inhibition of IgE receptor-triggered histamine and prostaglandin D2 release, apoptosis
Cell Type
Basophils, Eosinophils, Mast cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, Siglec Molecules
Antigen References

1. Bochner BS, et al. 2009. Clin. Exp. Allergy. 39:317.
2. Hudson SA, et al. 2009. J. Pharmacol. Exp. Ther. 330:608.
3. Nutku E, et al. 2005. Biochem. Biophys. Res. Commun. 336:918.

Gene ID
27181 View all products for this Gene ID
UniProt
View information about Siglec-8 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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