PE anti-mouse CD31 (PECAM-1) Antibody, CD31, W18222B

Pricing & Availability

Clone: W18222B (See other available formats)
Regulatory Status: RUO
Other Names: PECAM-1, EndoCAM
Isotype: Rat IgG2b, κ
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Product Citations: publications

W18222B_PE_CD31_Antibody_050120 — C57BL/6 mouse splenocytes were stained with CD3 Alexa Fluor® 647 and CD31 (clone W18222B) PE (left) or rat IgG2b, κ PE Isotype control (right).

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160203

25 µg

69€

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160204

100 µg

185€

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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α_v β₃ integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD31 transfected cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2876567 (BioLegend Cat. No. 160203)
AB_2876567 (BioLegend Cat. No. 160204)

Antigen Details

Structure: Ig superfamily, 130-140 kD
Distribution: Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
Function: Adhesion
Ligand/Receptor: CD38, α_V/β₃ integrin
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.

DeLisser HM, et al. 1994. Immunol Today. 15:490-5.

Newman PJ, et al. 1990. Science. 247:1219-22.
Gene ID: 18613 View all products for this Gene ID
UniProt: View information about CD31 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD31 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse CD31 (PECAM-1)	W18222B	FC
Purified anti-mouse CD31 (PECAM-1)	W18222B	FC,IHC-F
Alexa Fluor® 488 anti-mouse CD31 (PECAM-1) Antibody	W18222B	FC
APC anti-mouse CD31 (PECAM-1)	W18222B	FC
PE/Cyanine7 anti-mouse CD31 (PECAM-1)	W18222B	FC
FITC anti-mouse CD31 (PECAM-1)	W18222B	FC
PerCP/Cyanine5.5 anti-mouse CD31 (PECAM-1)	W18222B	FC
Pacific Blue™ anti-mouse CD31 (PECAM-1)	W18222B	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 05-01-2020

For Research Use Only. Not for diagnostic or therapeutic use.

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