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- Clone
- W20280E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Thy-1, Thy1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (clone 17A2) APC and anti-mouse CD90 (Thy1) (clone W20280E) PE (left) or rat IgG2a, κ PE isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 166407 | 25 µg | 92€ | ||||
| 166408 | 100 µg | 192€ | ||||
CD90, also known as Thy-1, is a 25-35 kD immunoglobulin superfamily member. It is expressed on hematopoietic stem cells, neurons, all thymocytes, and peripheral T cells, but not on B cells. CD90 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90 is involved in co-stimulation of lymphocyte proliferation and activation, and hematopoietic stem cell differentiation. CD90 has been shown to interact with CD45.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD90 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Precise lot-specific concentrations are provided for accurate titration due to the low recommended usage of this product. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.0075 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone W20280E can recognize both mouse CD90.1 (Thy1.1) and CD90.2 (Thy1.2) variants.
Antigen Details
- Structure
- Ig superfamily, 25-35 kD
- Distribution
-
Hematopoietic stem cells and neurons, thymocytes, T cells
- Function
- Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
- Ligand/Receptor
- CD45
- Cell Type
- Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Mesenchymal Stem Cells, Neurons, Thymocytes
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Craig W, et al. 1993. J Exp Med. 177:1331-42.
- Mayani H, et al. 1994. Blood. 83:2410-7.
- Gene ID
- 21838 View all products for this Gene ID
- UniProt
- View information about CD90 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD90 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD90 (Thy1) | W20280E | FC |
| PE anti-mouse CD90 (Thy1) | W20280E | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD90 (Thy1)
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (... -
PE anti-mouse CD90 (Thy1)
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (...
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