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PE anti-mouse RAE-1δ Antibody

Pricing & Availability
Clone
Charlotte d1.23 (See other available formats)
Regulatory Status
RUO
Other Names
Retinoid acid early inducible gene-1δ, RAE-1δ, RAE-1δ
Isotype
Mouse IgG1
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Product Citations
publications
ChD123_PE_042109.jpg
RAE-1δ transfected cells stained with Charlotte d1.23 PE
  • ChD123_PE_042109.jpg
    RAE-1δ transfected cells stained with Charlotte d1.23 PE
See PE spectral data
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133203 50 µg 98€
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Description

RAE-1δ is one of the five RAE-1 family, GPI-linked membrane protein consisting of alpha, beta, gamma, delta, and epsilon. They are strong homology within the family, related by 92%-95% sequence identity. They are distantly related to MHC class I proteins. RAE-1 proteins are abundantly expressed in fetal tissues, but not in normal adult tissue. They are constitutively expressed on some tumors and can be induced by retinoid acid. Its ligand NKG2D is found on NK cells, many transformed cell lines, and LPS-stimulated peritoneal macrophages. The interaction of NKG2D with RAE-1 transmits stimulatory signals to NK cells and other hematopoietic cells, leading to enhanced proliferation, cytokine secretion and target killing. They are involved in the regulation of innate and immune cytotoxic responses to tumors, pathogen-infected cells, and autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse RAE-1δ transfected RMA-S cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.015 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
  1. Lodoen M, et al. 2003. J. Exp. Med. 197:1245
  2. Carayannopoulos LN, et al. 2002. Eur J. Immunol.32 (3):597
RRID
AB_1595625 (BioLegend Cat. No. 133203)

Antigen Details

Structure
RAE is a family of GPI-linked membrane protein consisting of alpha, beta, gamma, delta, and epsilon. They are strong homology within the family, related by 92%-95% sequence identity. They are distantly related to MHC class I proteins.
Distribution
RAE-1 proteins are abundantly expressed in fetal tissues, but not in normal adult tissue. They are constitutively expressed on some tumors and can be induced by retinoid acid.
Ligand/Receptor
NKG2D
Biology Area
Immunology, Innate Immunity
Antigen References

1. Cerwenka A, et al. 2000. Immunity 12:721
2. Lodoen M, et al. 2003. J. Exp. Med. 197:1245
3. Diefenbach A, et al. 2001. Nature 413:165

Gene ID
66679 View all products for this Gene ID
UniProt
View information about RAE-1 delta on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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