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PE anti-rat CD43 Antibody

Pricing & Availability
Clone
W3/13 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, W3/13
Isotype
Mouse IgG1, κ
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Product Citations
publications
W3slash13_PE_081210
LOU rat splenocytes stained with W3/13 PE
  • W3slash13_PE_081210
    LOU rat splenocytes stained with W3/13 PE
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202812 100 µg 172€
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Description

CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and W3/13, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat thymocyte membrane glycoproteins
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections1 and paraffin-embedded sections4.

Application References
  1. Barclay AN. 1981. Immunology 42:593.
  2. Brown WRA, et al. 1981. Nature 289:456.
  3. Forbes RC, et al. 1983. Transplantation 36:681.
  4. Jung S, et al. 1994. Immunology 83:545.
Product Citations
  1. Liu P, et al. 2022. Exp Mol Med. 54:765. PubMed
  2. Person JM, et al. 2020. J Neuroimmunol. 577226:343. PubMed
  3. Bove GM et al. 2017. PloS one. 12(6):e0178407 . PubMed
  4. Guiteras J, et al. 2021. Int J Mol Sci. 22:. PubMed
  5. Hall JM, et al. 2021. Infect Immun. 89:e0030421. PubMed
  6. Hall JM, et al. 2021. Infect Immun. 89:e0034621. PubMed
  7. Bake S, et al. 2021. Brain Behav Immun. 98:86. PubMed
  8. Ruppert KA, et al. 2018. Sci Rep. 8:480. PubMed
RRID
AB_10642816 (BioLegend Cat. No. 202812)

Antigen Details

Structure
Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130kD
Distribution

Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.

Function
Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
Ligand/Receptor
CD54, Siglec-1
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238
  2. Killeen A, et al. 1987. EMBO J. 6(13):4029.
  3. Thurman EC, em>et al. 1998. Intl. Immunol. 10:691.
  4. Onami TM, et al. 2002. J. Immunol. 168:6022.
  5. Tong J, et al. 2004. J. Exp. Med. 199:1277.
  6. Jones AT, et al. 1994. J. Immunol. 153:3426.
Gene ID
20737 View all products for this Gene ID
UniProt
View information about CD43 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 06-18-2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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