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PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
D7 (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
D7_PECy7_091207
C57BL/6 mouse splenocytes stained with D7 PE/Cyanine7
  • D7_PECy7_091207
    C57BL/6 mouse splenocytes stained with D7 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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108113 25 µg 82€
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108114 100 µg 253€
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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The Ly-6A/E antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 

Application References
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Wu J, et al. 2022. Stem Cells Int. 2022:4249843. PubMed
  2. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  3. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  4. Moiseeva V, et al. 2023. Nature. 613:169. PubMed
  5. Huang D, et al. 2023. Exp Hematol Oncol. 12:11. PubMed
  6. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  7. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  8. Hong X, et al. 2022. Cell Stem Cell. 29:1298. PubMed
  9. Pioli KT, et al. 2023. iScience. 26:106223. PubMed
  10. Bobadilla Muñoz M, et al. 2023. Front Cell Dev Biol. 11:1128534. PubMed
  11. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  12. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  13. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  14. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  15. Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
  16. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  17. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  18. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  19. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  20. DellOrso S, et al. 2021. STAR Protocols. 2(2):100451. PubMed
  21. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  22. Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed
  23. Campanario S, et al. 2021. Front Cell Dev Biol. 8:620409. PubMed
  24. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  25. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  26. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  27. Arai F, et al. 2020. Cell Systems. 11(6):640-652.e5. PubMed
  28. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  29. Ankawa R, et al. 2021. Developmental Cell. . PubMed
  30. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  31. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  32. Solanas G, et al. 2017. Cell. 170:678. PubMed
  33. Gross ETE, et al. 2018. Oncoimmunology. 8:e1404212. PubMed
  34. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  35. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  36. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  37. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  38. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  39. Dong S, et al. 2021. Nature. 591:117. PubMed
  40. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  41. Telford W, et al. 2017. Cytometry A. 91:314. PubMed
  42. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  43. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  44. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  45. Smith E, et al. 2009. J Immunol. 183:5685. PubMed
  46. Cho K, et al. 2021. iScience. 24:103117. PubMed
  47. Dagher T, et al. 2021. J Exp Med. 218:. PubMed
  48. García-Prat L, Muñoz-Cánoves P, Martínez-Vicente 2017. Muscle Stem Cells. 10.1007/978-1-4939-6771-1_14. PubMed
  49. Zaro BW, et al. 2020. eLife. 9:00. PubMed
  50. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  51. Duan Y, et al. 2018. Stem Cells. 36:1430. PubMed
  52. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  53. Gough D, et al. 2014. Blood. 124:2252. PubMed
  54. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  55. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  56. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  57. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  58. Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed
  59. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  60. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  61. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  62. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  63. Giraddi R, et al. 2015. Nat Commun. 6: 8487. PubMed
  64. Kang M, et al. 2021. Front Cell Dev Biol. 8:596622. PubMed
  65. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  66. Azazmeh N, et al. 2020. Nat Commun. 2.340972222. PubMed
  67. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  68. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  69. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  70. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  71. Zheng Z, et al. 2021. Nat Commun. 12:6202. PubMed
  72. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  73. Liu F, et al. 2021. Cell Reports. 35(10):109225. PubMed
  74. Jin Y, et al. 2020. PeerJ. 8:e10374. PubMed
  75. Yosef R, et al. 2016. Nat Commun. 7: 11190. PubMed
  76. Christoforou N, et al. 2013. PLoS One. 8:65963. PubMed
  77. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  78. Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed
  79. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  80. Chavez JS, et al. 2022. Cells. 11:. PubMed
  81. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  82. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  83. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  84. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  85. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  86. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  87. Zhang B, et al. 2021. Nature. 599:471. PubMed
  88. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  89. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  90. Del Barco Barrantes I, et al. 2018. Stem Cell Reports. 10:257. PubMed
  91. Hacein-Bey-Abina S, et al. 2020. Exp Hematol. 88:15. PubMed
  92. Man N, et al. 2021. JCI Insight. 6:. PubMed
  93. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  94. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  95. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  96. Youssif C, et al. 2018. EMBO Mol Med. 10:e8403. PubMed
RRID
AB_493596 (BioLegend Cat. No. 108113)
AB_493596 (BioLegend Cat. No. 108114)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org
Go To Top Version: 3    Revision Date: 07-11-2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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