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PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
E13-161.7 (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
E13-161dot7_PECy7_091207
C57BL/6 mouse splenocytes stained with E13-161.7 PE/Cyanine7
  • E13-161dot7_PECy7_091207
    C57BL/6 mouse splenocytes stained with E13-161.7 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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122513 25 µg 81€
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122514 100 µg 249€
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Description

Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mouse pre-T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7

Application References
  1. Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
  2. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
  3. van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
  4. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
  5. Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
  6. Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
  7. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  8. Artinger EL, et al. 2013. PNAS. 110:12000. PubMed
Product Citations
  1. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  2. Zachara M, et al. 2022. EMBO J. 41:e108206. PubMed
  3. Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed
  4. Li X, et al. 2022. Cell. 185:1709. PubMed
  5. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  6. Rahman SMT, et al. 2022. Cell Rep. 41:111682. PubMed
  7. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  8. Levra Levron C, et al. 2023. Nat Cell Biol. 25:740. PubMed
  9. Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed
  10. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  11. Guo B, et al. 2018. Nat Med. 24:360. PubMed
  12. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  13. Hara T, et al. 2012. J Immunol. 189:1577. PubMed
  14. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  15. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  16. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  17. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  18. Bues J, et al. 2022. Nat Methods. 19:323. PubMed
  19. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  20. Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed
  21. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  22. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  23. Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed
  24. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  25. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  26. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  27. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  28. Thürmer M, et al. 2022. Nat Commun. 13:2982. PubMed
  29. Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
  30. Muzumdar S, et al. 2019. Am J Pathol. 189:568. PubMed
  31. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  32. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  33. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
RRID
AB_756198 (BioLegend Cat. No. 122513)
AB_756198 (BioLegend Cat. No. 122514)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 07-11-2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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