Human peripheral blood lymphocytes were stained with biotinylated CD3, followed by SAV- PE/Fire™ 700 (filled histogram), or biotinylated mouse IgG1 isotype control, followed with SAV- PE/Fire™ 700 (open histogram).
Human peripheral blood lymphocytes were stained with biotinylated CD3, followed by SAV- PE/Fire™ 700 (filled histogram), or biotinylated mouse IgG1 isotype control, followed with SAV- PE/Fire™ 700 (open histogram).
Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire™ 700 has a maximum excitation of 565 nm and a maximum emission of 695 nm.
Excitation Laser
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
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