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PerCP/Cyanine5.5 anti-human CD5 Antibody

Pricing & Availability
Clone
L17F12 (See other available formats)
Regulatory Status
RUO
Workshop
II T7
Other Names
Leu-1, Ly-1, T1, Tp67, Leu1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
L17F12_PerCPCy5.5_CD5_Antibody_FC_102714
Human peripheral blood lymphocytes were stained with CD5 (clone L17F12) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • L17F12_PerCPCy5.5_CD5_Antibody_FC_102714
    Human peripheral blood lymphocytes were stained with CD5 (clone L17F12) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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364005 25 tests 128€
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364006 100 tests 306€
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Description

CD5, also known as Leu-1, Ly-1 and T1, is a 67 kD single chain type I glycoprotein that is a member of the scavenger receptor superfamily. CD5 is expressed on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-CLL), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions. One of its ligands is CD72.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human T-acute lymphoblastic leukemia (ALL) cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats): immunoprecipitation1,3,4 and immmunohistochemical staining of frozen and formalin-fixed paraffin-embedded sections2,3,5.

Application References
  1. McAlister MS, et al. 1998. Protein Eng. 11:847. (IP)
  2. Butmarc JR, et al. 1998. Am. J. Clin. Pathol. 109:682. (IHC)
  3. Engleman EG, et al. 1981. Proc. Natl. Acad. Sci. USA 78:1791. (IHC, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310. (FC, IP)
  5. Warnke R and Levy R. 1980. J. Histochem. Cytochem. 28:771. (IHC)
Product Citations
  1. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  2. van der Heiden M, et al. 2020. Scand J Immunol. e13005:. PubMed
RRID
AB_2564502 (BioLegend Cat. No. 364005)
AB_2564502 (BioLegend Cat. No. 364006)

Antigen Details

Structure
Scavenger receptor superfamily, 67 kD
Distribution

Thymocytes, T cells, and subsets of B and dendritic cells.

Function
Modulates TCR and BCR signaling, involved in thymocyte maturation and T-B cell interaction
Ligand/Receptor
CD72
Cell Type
B cells, Dendritic cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Kipps T. 1989. Adv. Immunol. 47:117.
2. Resnick D, et al. 1994. Trends Biochem. Sci. 1:5.
3. Wood GS and Freudenthal PS. 1992. Am. J. Pathol. 141:789.

Gene ID
921 View all products for this Gene ID
UniProt
View information about CD5 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 05-24-2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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