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PerCP/Cyanine5.5 anti-human CD85k (ILT3) Antibody

Pricing & Availability
Clone
ZM4.1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin-like transcript (ILT) 3, CD85k, Leukocyte Immunoglobulin-Like Receptor subfamily B member 4 (LILRB4), Leukocyte Immunoglobulin-like Receptor 5 (LIR-5)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
ZM41_PerCPCyanine55_CD85k_Antibody_012919
Human peripheral blood lymphocytes were stained with CD123 APC and CD85k (clone ZM4.1) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
  • ZM41_PerCPCyanine55_CD85k_Antibody_012919
    Human peripheral blood lymphocytes were stained with CD123 APC and CD85k (clone ZM4.1) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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333014 100 tests 306€
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Description

CD85k, known as Immunoglobulin-like transcript (ILT) 3, is an inhibitory receptor expressed by dendritic cells and a subset of white blood cells called monocytes.  ILT3, also known as Leukocyte Immunoglobulin-Like Receptor subfamily B member 4 (LILRB4) and Leukocyte Immunoglobulin-like Receptor 5 (LIR-5), is a type I membrane protein that contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses. Expression of ILT3 is up-regulated on tolerogenic dendritic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
ILT3 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Product Citations
  1. Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed
RRID
AB_2565559 (BioLegend Cat. No. 333014)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

Monocytes, Macrophages, and Dendritic Cells

Cell Type
Dendritic cells, Macrophages, Monocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Cella M, et al. 1997. J Exp Med. 185:1743-51.
  2. Penna G, et al. 2005. Blood. 5:2044.
Gene ID
11006 View all products for this Gene ID
UniProt
View information about CD85k on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 01-29-2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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