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PerCP/Cyanine5.5 anti-human CXCL10 (IP-10) Antibody

Pricing & Availability
Clone
J034D6 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon gamma induced protein-10 (IP-10)
Isotype
Mouse IgG2a, κ
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Product Citations
publications
J034D6_PERCP_CYANINE55_CXCL10_Antibody_020422
Human peripheral blood monocytes were stimulated with IFN-γ (1500U/mL) for 24 hours (in the presence of monensin), fixed, and permeabilized. Cells were then stained with anti-human CD14 and anti-human CXCL10 (clone J034D6) PerCP/Cyanine5.5 (left) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • J034D6_PERCP_CYANINE55_CXCL10_Antibody_020422
    Human peripheral blood monocytes were stimulated with IFN-γ (1500U/mL) for 24 hours (in the presence of monensin), fixed, and permeabilized. Cells were then stained with anti-human CD14 and anti-human CXCL10 (clone J034D6) PerCP/Cyanine5.5 (left) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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519509 25 tests 132€
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519510 100 tests 310€
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Description

CXCL10, also known as IP-10, is a 8.7 kD chemokine. It belongs to the CXC chemokine family. It is expressed by monocytes, fibroblasts, and endothelial cells in response to IFNγ. It can interact with CXCR3 and plays an important role in immune cell trafficking, particularly of T cells, dendritic cells, and NK cells. In addition, it alters expression of adhesion molecules.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC – Quality tested 
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
RRID
AB_2922627 (BioLegend Cat. No. 519509)
AB_2922627 (BioLegend Cat. No. 519510)

Antigen Details

Structure
CXC chemokine family, 8.7 kD
Distribution

Monocytes, endothelial cells, and fibroblasts

Function
Interacts with CXCR3 and serves as a chemoattractant for T cells, dendritic cells, monocytes/macrophages, and NK cells
Ligand/Receptor
CXCR3
Cell Type
B cells, Dendritic cells, Endothelial cells, Fibroblasts, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Luster AD, et al. 1987. Proc. Natl. Acad. Sci. USA 84:2868. 
2. Kaplan G, et al. 1987. J. Exp. Med. 166:1098. 
3. Sarris AH, et al. 1993. J. Exp. Med. 178:1127.

Gene ID
3627 View all products for this Gene ID
UniProt
View information about CXCL10 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 02-04-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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