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PerCP/Cyanine5.5 anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
W18272D (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Rat IgG2b, κ
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Product Citations
publications
W18272D_PERCP-CYA55_IFN-G_Antibody_122823
C57BL/6 mouse splenocytes were treated with (left) or without (right) Cell Activation Cocktail (with Brefeldin A) (Cat. Nos. 423303 and 423304) for 5 hours. Cells were then surface stained with anti-mouse CD3 (clone 145-2C11) FITC, then fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat: 426803) and intracellularly stained with anti-mouse IFN-γ (clone W18272D) PerCP/Cyanine5.5.
  • W18272D_PERCP-CYA55_IFN-G_Antibody_122823
    C57BL/6 mouse splenocytes were treated with (left) or without (right) Cell Activation Cocktail (with Brefeldin A) (Cat. Nos. 423303 and 423304) for 5 hours. Cells were then surface stained with anti-mouse CD3 (clone 145-2C11) FITC, then fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat: 426803) and intracellularly stained with anti-mouse IFN-γ (clone W18272D) PerCP/Cyanine5.5.
Compare all formats See PerCP/Cyanine5.5 spectral data
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163509 25 µg 88€
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163510 100 µg 268€
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Description

IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
RRID
AB_3097440 (BioLegend Cat. No. 163509)
AB_3097440 (BioLegend Cat. No. 163510)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Distribution

Tregs

Function
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Ligand/Receptor
Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Antigen References
1. Zaidi et al. 2011, Clin Cancer Res,17(19):6118-24
2. Castro et al. 2018, Front Immunol, 9:847
3. Alspach et al. 2019, Cold Spring Harb Perspect Biol, 11(3):a028480
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 12-28-2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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