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PerCP/Cyanine5.5 anti-mouse IgG2a Antibody

Pricing & Availability
Clone
RMG2a-62 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2a
Isotype
Rat IgG
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Product Citations
publications
RMG2A_PerCpCy5.5_IgG2a_Antibody_FC_041013
Human peripheral blood lymphocyte were stained with purified anti-human CD3 (clone HIT3a, filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG2a (clone RMG2a-62) PerCP/Cyanine5.5.
  • RMG2A_PerCpCy5.5_IgG2a_Antibody_FC_041013
    Human peripheral blood lymphocyte were stained with purified anti-human CD3 (clone HIT3a, filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG2a (clone RMG2a-62) PerCP/Cyanine5.5.
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
407111 25 µg 72€
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407112 100 µg 156€
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Description

The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References
  1. Gavin AL, et al.2006. Science 314:1936.
  2. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
Product Citations
  1. Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed
  2. Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed
  3. Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed
  4. Hayashi Y, et al. 2017. J Biol Chem. 292(3):1122-1141. PubMed
RRID
AB_2562007 (BioLegend Cat. No. 407111)
AB_2562007 (BioLegend Cat. No. 407112)

Related FAQs

IgG2a gene is deleted in some mouse strains, which ones are they?

IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 04-18-2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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