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PhosphoPair Lck (Tyr394) Antibody Set

Pricing & Availability
Clone
LCK-01; A18002D;
Regulatory Status
RUO
Other Names
LSK; 56lck; LCK Proto-Oncogene, Src Family Tyrosine Kinase; p56LCK; Pp58Lck T Cell-Specific Protein Tyrosine Kinase
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Product Citations
publications
PhosphoPair_Lck_Antibody_Set_081120.png
Whole cell extracts (15 µg protein) from serum starved Jurkat cells untreated (-) or treated (+) with 5 mM H2O2 for 3 minutes were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2500 dilution) of purified anti-Lck Phospho (Tyr394) antibody (clone A18002D) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal Lck loading was confirmed by probing membranes with purified anti-Lck antibody (clone LCK-01) at 1.0 µg/mL (1:500 dilution). Lane M: Molecular weight marker.
  • PhosphoPair_Lck_Antibody_Set_081120.png
    Whole cell extracts (15 µg protein) from serum starved Jurkat cells untreated (-) or treated (+) with 5 mM H2O2 for 3 minutes were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2500 dilution) of purified anti-Lck Phospho (Tyr394) antibody (clone A18002D) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal Lck loading was confirmed by probing membranes with purified anti-Lck antibody (clone LCK-01) at 1.0 µg/mL (1:500 dilution). Lane M: Molecular weight marker.
  • PhosphoPair_Lck_Antibody_Set_2_081120.png
    Whole cell extracts (15 µg protein) from Jurkat and J.Cam1.6 cells untreated (-) or treated (+) with 1 µM staurosporine for 3 hours were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2500 dilution) of purified anti-Lck Phospho (Tyr394) antibody (clone A18002D) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal Lck loading was confirmed by probing membranes with purified anti-Lck antibody (clone LCK-01) at 1.0 µg/mL (1:500 dilution). Lane M: Molecular weight marker.
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Description

The Src family tyrosine kinase p56Lck (Lck) is a non-receptor tyrosine kinase that plays a critical role in T cell selection and maturation within the thymus, and also in the function of mature T cells. Lck, which is constitutively bound to cytosolic domains of CD4 and CD8 surface receptors, plays an essential role in T cell receptor (TCR) signaling. Engagement of the TCR with peptide antigen-loaded MHC complexes results in the recruitment of CD4- and CD8-bound Lck to the TCR/CD3 signaling complex. Lck then transphosphorylates TCR-gamma chains and CD3 subunits, thereby activating the TCR/CD3 signaling pathway and leading to the recruitment and subsequent phosphorylation of Zap70 by Lck. Lck also plays an important role in interleukin-2 signaling that regulates the T cell proliferative response. Phosphorylation of Lck by CSK at tyrosine 505 negatively regulates the kinase, and is proposed to generate a closed, inactive conformation of the protein. Conversely, phosphorylation at tyrosine 394 in the activation loop of Lck greatly stimulates enzymatic activity. In many types of cancer, Lck is a proliferative and anti-apoptotic driver, and has been proposed as a target for therapeutic intervention.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Product Application Clone Size Reactivity Isotype
Purified anti-Lck Antibody WB, ICC, IP LCK-01 25 μg Human Mouse IgG1, κ
Purified Lck Phospho (Tyr394) Antibody WB, ICC, ICFC A18002D 25 μg Human, Mouse Mouse IgG1, κ


* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Verified Reactivity
Human
Immunogen
Please refer to individual product datasheets for immunogen information
Formulation
Phosphate-buffered solutions, pH 7.2, containing 0.09% sodium azide
Preparation
The antibodies were purified by affinity chromatography.
Storage & Handling
The antibody solutions should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:

Clone LCK-01: 1.0 µg/mL
Clone A18002D: 0.1 - 1.0 µg/mL

It is recommended that the reagent be titrated for optimal performance for each application.

For recommended usage information for additional applications, please refer to the datasheet of the individual products.

Antigen Details

Structure
Lck is a 509 amino acid protein with a predicted molecular weight of ~58 kD.
Distribution

T cells/Plasma membrane-associated

Function
T cell receptor signaling
Antigen References
  1. Phillipsen L, et al. 2017. Sci Signal. 10:eaaf4736.
  2. Moogk D, et al. 2016. J Immunol. 197:644.
  3. Klammt C, et al. 2015. Nat Immunol. 16:961.
  4. Cancer Genome Atlas Network. 2015. Cell. 161:1681.
  5. Casas J, et al. 2014. Nat Commun. 5:5624.
  6. McNeill L, et al. 2007. Immunnity. 27:425.
  7. Lefebvre DC, et al. 2003. Biochim Biophys Acta. 1650:40.
  8. Kabouridis PS. et al. 2003. Biochem J. 371:907.
  9. Abraham N and Veillette 1990. Mol. Cell. Biol. 10:5197.
Gene ID
3932 View all products for this Gene ID
UniProt
View information about Lck on UniProt.org
Go To Top Version: 1    Revision Date: 08-11-2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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