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Purified anti-Aldolase C Antibody

Pricing & Availability
Clone
A18070A (See other available formats)
Regulatory Status
RUO
Other Names
Aldolase, fructose-biphosphate C, ALDC, Aldolase C, Aldolase 3, Fructoaldolase C
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A. A18070A_PURE_ALDOLASE-C_WB_060623
Protein extracts (15 µg total protein) from the indicated cell lines and tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-Aldolase C (clone A18070A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. A18070A_PURE_ALDOLASE-C_WB_060623
    Protein extracts (15 µg total protein) from the indicated cell lines and tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-Aldolase C (clone A18070A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. A18070A_PURE_ALDOLASE-C_IHCP_060623
    IHC staining of purified anti-Aldolase C (clone A18070A) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using 1X Tris-EDTA, pH 9.0, the tissue was incubated without (panel A) and with (panel B) 1 µg/mL of purified anti-Aldolase C (clone A18070A) overnight in 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 20X objective. Scale bar: 50 µm
  • C. A18070A_PURE_ALDOLASE-C_ICC_060623
    Cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) and permeabilized with methanol for 10 minutes, and then blocked with 5% FBS for 1 hour at room temperature. A549 cells (low expression control, panel B and D) and U87-MG cells (positive control, panel A and C) were then stained with 5.0 µg/mL of purified mouse IgG2b, κ isotype control (Cat. No. 402202) (panels A and B) or purified anti-Aldolase C (clone A18070A), followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 60X objective. Scale bar: 50 µm
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615651 25 µg 104€
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615652 100 µg 332€
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Description

Aldolase C is one of the three aldolase isozymes and plays a key role in ATP biosynthesis. It participates in glycolysis and gluconeogenesis by catalyzing the conversion of fructose-1-6-biphosphate to glyceraldehyde-3-phosphate, or glyceraldehyde, and di-hydroxy-acetone-phosphate. In addition, it is part of the stress-response pathway induced by hypoxia. It has been found that Aldolase C is upregulated in the brains of schizophrenia patients. Moreover, inactivation of Aldolase C by oxidation has been reported in mild cognitive impairment (MCI) and Alzheimer’s disease (AD).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Aldolase C
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2941622 (BioLegend Cat. No. 615651)
AB_2941622 (BioLegend Cat. No. 615652)

Antigen Details

Structure
Aldolase C is a 364 amino acid protein with an expected molecular weight of 39 kD.It is a member of the three aldolase isozymes. Aldose C shares 68% identity with Aldolase B and 78% with Aldolase A. The catalytic site is highly conserved in all three isoforms.
Distribution

Although Aldolase C is widely expressed, it is predominantly expressed in the hippocampus, Purkinje cells, neurons of the brain, and smooth muscle.

Function
Glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References
  1. Chen X, et al. 2022. Nature. 605:722-727.
  2. Chang YC, et al. 2018. Trends Endocrinol Metab. 28:549-559.
  3. Juszczak GR, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 82:136.
  4. Lai GE, et al. 2022. Gland Surg. 11:826-836.
Gene ID
230 View all products for this Gene ID
UniProt
View information about Aldolase C on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06-06-2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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