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Purified anti-α-Synuclein, 80-96 Antibody

Pricing & Availability
Clone
A15115A (See other available formats)
Regulatory Status
RUO
Other Names
Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP), PARK1, NACP, PD1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A15115A_Purified_alpha_synuclein_Antibody_1_012122
IHC staining of purified anti-α-Synuclein, 80-96 antibody (clone A15115A) on formalin-fixed paraffin-embedded Parkinson's disease human brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • A15115A_Purified_alpha_synuclein_Antibody_1_012122
    IHC staining of purified anti-α-Synuclein, 80-96 antibody (clone A15115A) on formalin-fixed paraffin-embedded Parkinson's disease human brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • A15115A_Purified_alpha_synuclein_Antibody_2_012122
    Western blot of purified anti-α-Synuclein, 80-96 antibody (clone A15115A), and isotype-matched IgG1 control, demonstrating the binding specificity of clone A15115A to endogenous and recombinant human α-synuclein. Lane 1: 50 ng of recombinant human α-synuclein; Lane 2: 50 ng of recombinant human pS87 α-synuclein; Lane 3: 25 µg of normal human brain lysate. Blots were incubated with 1.0 µg/mL of each primary antibody at room temperature for two hours, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
  • A15115A_Purified_alpha_synuclein_Antibody_3_012122
    Direct ELISA of purified anti-α-Synuclein antibody, 80-96 (clone A15115A), binding to plate-immobilized recombinant human unmodified and pS87 α-synuclein. ELISA was performed by coating wells with 150 ng of each recombinant α-synuclein protein for two hours at 37°C. The wells were then incubated with the primary antibody at 37°C for one hour, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. 3, 3', 5, 5' tetramethylbenzidine (TMB) was used as the detection system.
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848301 25 µg 72€
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848302 100 µg 175€
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Description

Alpha-synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of α-synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms include, α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-Conjugated synthetic peptide that included amino acid residues 80-96 of human α-synuclein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Direct ELISA, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. For Direct ELISA, the suggested use of this reagent is 0.02 - 3.0 per ml. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not cross-react with ß or ?-synucleins using ELISA and WB as detection method.

Product Citations
  1. Gustavsson N, et al. 2021. Acta Neuropathol Commun. 9:153. PubMed
RRID
AB_2650687 (BioLegend Cat. No. 848301)
AB_2650687 (BioLegend Cat. No. 848302)

Antigen Details

Structure
α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
Distribution

Tissue distribution: Primarily nervous system, but lower expression in other tissues such as blood and skin.
Cellular distribution: Cytoskeleton, cytosol,  lysosome, mitochondria, nucleus, plasma membrane, and extracellular.

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein, Phospho-Proteins
Antigen References

1. Mor DE, et al. 2016. Neurobiol Dis. 88:66. PubMed
2. Jucker M & Walker LC. 2013. Nature. 501(7465):45. PubMed
3. Bartels T, et al. 2011. Nature. 477(7362): 107. PubMed 
4. Devine MJ, et al. 2011. Mov Disord. 26:2160. PubMed

Gene ID
6622 View all products for this Gene ID
UniProt
View information about alpha-Synuclein 80-96 on UniProt.org
Go To Top Version: 3    Revision Date: 12-21-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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