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Purified anti-ASCL1 (MASH1) Antibody

Pricing & Availability
Clone
W23121G (See other available formats)
Regulatory Status
RUO
Other Names
Achaete-Scute Family BHLH Transcription Factor 1, Achaete-Scute Complex (Drosophila) Homolog-Like 1, HASH1, BHLHa46, ASH-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W23121G_PURE_ASCL1_WB_031325
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-ASCL1 (MASH1) (clone W23121G) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W23121G_PURE_ASCL1_WB_031325
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-ASCL1 (MASH1) (clone W23121G) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W23121G_PURE_ASCL1_ICC_031325
    HeLa cells (negative cell line, panel A) and NCI-H146 cells (positive cell line, panel B) were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-ASCL1 (MASH1) (clone W23121G), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • C. W23121G_PURE_ASCL1_ICC-2_031325
    HeLa cells (negative cell line, panel A) and NCI-H146 cells (positive cell line, panel B) were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-ASCL1 (MASH1) (clone W23121G), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • d. W23121G_PURE_ASCL1_ICFC_031325
    NCI-H146 cells (positive cell line, filled histogram) and Jurkat cells (negative cell line, open histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-ASCL1 (MASH1) (clone W23121G) or Purified Rat IgG2a, κ Isotype Control (Cat. No. 400502) (open histogram, dashed line, representative for both positive and negative cell lines) followed by Alexa Fluor® 647 Goat anti-rat IgG antibody (Cat. No. 405416).
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673051 25 µg 132€
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673052 100 µg 332€
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Description

ASCL1 (Achaete-Scute Family BHLH Transcription Factor 1), also known as MASH1, is a transcription factor that plays a crucial role in neurogenesis, particularly in the development of the nervous system. ASCL1 is a member of the basic helix-loop-helix (BHLH) transcription factor family and acts by binding to specific E-box sequences in the DNA to regulate the expression of target genes that are necessary for the cell fate determination, differentiation, and development. The protein is expressed in various tissues during development, including the developing spinal cord, cortex, and hindbrain. In the neural tube, ASCL1 influences the differentiation of neural progenitors into various types of neurons, including dopaminergic, serotonergic, and other interneurons. The loss of ASCL1 function during development can lead to neurological disorders such as Hirschsprung disease, congenital central hypoventilation syndrome, dysautonomia. ASCL1 has been found to be involved in the progression of certain cancers, such as lung and neuroendocrine tumors. In these tumors, ASCL1 is often overexpressed and contributes to tumorigenesis by promoting the survival and proliferation of cancer cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human ASCL1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Fixation/permeabilization with True-Phos™ Perm Buffer (Cat. No. 425401). Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended.

Antigen Details

Structure
Contain a basic helix-loop-helix (BHLH) domain. This protein consists of 236 amino acids with a predicted molecular weight of 25.5 kD.
Distribution

Expressed in brain, liver, and pituitary gland. Subcellular localization is in the nucleus.

Function
Transcription factor that plays a key role in neuronal differentiation
Interaction
Form heterodimer with TCF3 and bind to DNA
Cell Type
Neurons
Biology Area
Neuroscience, Neuroscience Cell Markers, Synaptic Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Pattyn A, et al. 2004. Nat Neurosci. 7:589-95.
  2. Castro DS, et al. 2011. Genes Dev. 25:930-45.
  3. Chanda S, et al. 2014. Stem Cell Reports. 3:282-96.
Gene ID
429 View all products for this Gene ID
UniProt
View information about ASCL1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03-13-2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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