- Clone
- W18024A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Branched Chain Amino Acid Transanimase 1, BCT1, BCATC, PNAS121, Protein ECA39
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
938001 | 25 µg | 81€ | ||||
938002 | 100 µg | 203€ |
BCAT1 is an aminotransferase that catalyzes the catabolism of the branched-chain amino acids leucine, isoleucine, and valine, making it an important regulator of the TCA cycle. BCAT1 also plays a role in cell cycle progression, differentiation, and apoptosis. Clinical disorders involved with mutations in the BCAT1 gene include hypervalinemia and hyperleucine-isoleucinemia.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full-length recombinant human BCAT1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone failed to immunoprecipitate BCAT1 from NTERA-2 lysates.
This clone was tested for immunocytochemistry on NTERA-2 cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in dim staining of BCAT1. We therefore do not recommend the clone for this application. - RRID
-
AB_2876757 (BioLegend Cat. No. 938001)
AB_2876757 (BioLegend Cat. No. 938002)
Antigen Details
- Structure
- BCAT1 is a 386 amino acid protein with a predicted molecular weight of 43 kD.
- Distribution
-
Ubiquitously expressed/Cytosolic
- Function
- Aminotransferase enzyme, amino acid catabolism
- Cell Type
- Macrophages
- Biology Area
- Cell Biology, Mitochondrial Function
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- Papathanassiu AE, et al. 2017. Nat. Commun. 8:16040.
- Xu M, et al. 2016. Oncol Lett. 12:2648-2656.
- Zhou W, et al. 2013. Mol Cancer. 12:53.
- Wegermann K, et al. 2018. PLoS One. 13:e0204308.
- Hattori A, et al. 2017. Nature. 545:500-504.
- Gene ID
- 586 View all products for this Gene ID
- UniProt
- View information about BCAT1 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All BCAT1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-BCAT1 Antibody | W18024A | WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-BCAT1 Antibody
Whole cell lysates (15 µg) from NTERA-2, Daudi, and indicate...
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