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Purified anti-BRST-2 (GCDFP-15) Antibody

Pricing & Availability
Clone
D6 (See other available formats)
Regulatory Status
RUO
Other Names
Prolactin-inducible protein (PIP), secretory actin-binding protein, gross cystic disease fluid protein 15, BRST2, GCDFP15, GCDFP-15, GPIP4
Isotype
Mouse IgG2a, κ
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Product Citations
publications
D6_PURE_BRST-2_Antibody_IHC-P_022819
IHC staining of anti-BRST-2 (GCDFP-15) antibody (clone D6) on formalin-fixed paraffin-embedded human breast tumor tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 0.2 µg/ml for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Kit (Multi-Species) was used for detection followed by counterstaining with hematoxylin.
  • D6_PURE_BRST-2_Antibody_IHC-P_022819
    IHC staining of anti-BRST-2 (GCDFP-15) antibody (clone D6) on formalin-fixed paraffin-embedded human breast tumor tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 0.2 µg/ml for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Kit (Multi-Species) was used for detection followed by counterstaining with hematoxylin.
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915106 100 µg 184€
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Description

The BRST-2 antigen is expressed by apocrine sweat glands, eccrine glands (variable), minor salivary glands, bronchial glands, metaplasmic epithelium of the breast, benign sweat gland tumors of the skin and the serous cells of the submandibular gland. Breast carcinomas (primary and metastatic lesions) with apocrine features express the BRST-2 antigen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated against Gross Cystic Disease Fluid Protein 15.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 1.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The BRST-2 clone D6 recognizes a 15 kD glycoprotein known as Gross Cystic Disease Fluid Protein-15 (GCDFP-15). Antigen retrieval is not required. The BRST-2 epitope is heat-sensitive; paraffin sections that have been heat fixed at 60°C may lose antibody binding sites.

Application References
  1. Yoshimura Y, et al. 2011. Jpn. J. Clin. Oncol. 41:358. (IHC) PubMed
  2. Fan CY, et al. 2000. Am. J. Surg. Pathol. 24(4):579. PubMed
  3. Wick MR, et al. 1989. Hum. Pathol. 20(3):281. PubMed
  4. Mazoujian G, et al. 1984. Am. J. Surg. Pathol. 8(1):43. PubMed
  5. Mazoujian G, et al. 1983. Am. J. Pathol. 110:105. PubMed
  6. Brown RW, et al. 1997. Am. J. Clin. Path. 107:12. PubMed
RRID
AB_2616964 (BioLegend Cat. No. 915106)

Antigen Details

Structure
BRST-2 (GCDFP-15) is a 17 kD protein.
Distribution

Extracellular and nucleus.

Function
Diseases associated with BRST-2 include florid papillomatosis of the nipple and breast signet ring cell adenocarcinoma. Interacts with AZGP1.
Biology Area
Cancer Biomarkers, Cell Biology
Gene ID
5304 View all products for this Gene ID
UniProt
View information about BRST-2 on UniProt.org
Go To Top Version: 1    Revision Date: 06-10-2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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