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Purified anti-CD38 Antibody

Pricing & Availability
Clone
W22190F (See other available formats)
Regulatory Status
RUO
Other Names
cluster of differentiation 38 (CD38), ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (ADPRC1/cADPR hydrolase 1), 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase
Isotype
Rat IgG2a, κ
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Product Citations
publications
A. W22190F_PURE_CD38_WB_022924
Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of Purified anti-CD38 (clone W22190F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W22190F_PURE_CD38_WB_022924
    Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of Purified anti-CD38 (clone W22190F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W22190F_PURE_CD38_IHC-P_022924
    IHC staining of Purified anti-CD38 (clone W22190F) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Purified anti-CD38 (clone W22190F) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • C. W22190F_PURE_CD38_ICC_022924
    MCF-7 cells (negative cell line, panel A) and Daudi cells (positive cell line, panel B) were fixed and permeabilized with 100% ice-cold methanol for 10 minutes and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 1.0 µg/mL of Purified anti-CD38 (clone W22190F), followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
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632351 25 µg 136€
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632352 100 µg 364€
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Description

CD38 is a widely-expressed, multifunctional protein whose role is best studied in the immune system, where it is expressed at varying levels in multiple immune cell types including lymphocytes, monocytes/macrophages, dendritic cells and NK cells. CD38 functions in cell signaling via protein-protein interactions with cell membrane proteins in other immune cell types. For example, CD38 interacts with the T cell receptor complex in T cells and the B cell co-receptor complex in B cells. CD38 also binds to CD31/PECAM in blood vessels to mediate lymphocyte adhesion. Additionally, CD38 has intrinsic enzymatic activity, catalyzing the synthesis of ADP ribose, cyclic ADP ribose, or nicotinamide from NAD. Due to the influence the synthesized molecules have on calcium dynamics, CD38 plays a role in diverse physiological processes, including regulating insulin secretion and modulation of neurotransmitter release.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human CD38
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone W22190F) is not suitable for flow cytometry (FC) or intracellular flow cytometry (ICFC).

For immunohistochemistry (IHC), we recommend antigen retrieval with either Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) or sodium citrate (Cat. No. 928502).

For immunocytochemistry (ICC), we recommend fixation/permeabilization with either 100% ice-cold methanol, or fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 0.5% Triton-X or 100% ice-cold methanol.

RRID
AB_3097610 (BioLegend Cat. No. 632351)
AB_3097610 (BioLegend Cat. No. 632352)

Antigen Details

Structure
CD38 is a 300 amino acid protein with a predicted molecular weight of 34.3 kD
Distribution

Cell surface/plasma membrane

Function
Receptor/co-receptor, enzyme
Interaction
CD16, T cell receptor, B cell receptor, class II MHC, CD31
Cell Type
B cells, Dendritic cells, NK cells, Plasma cells, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Glaria E, et al. 2020. Cells. 9:228.
  2. Piedra-Quintero ZL, et al. 2020. Front Immunol. 11:597959.
  3. Li Y, et al. 2020. Future Oncol. 16:2853-2861.
Gene ID
952 View all products for this Gene ID
UniProt
View information about CD38 on UniProt.org
Go To Top Version: 1    Revision Date: 03-01-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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