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Purified anti-Centrin 2 (Caltractin) Antibody

Pricing & Availability
Clone
W16110A (See other available formats)
Regulatory Status
RUO
Other Names
Caltractin isoform 1, CETN2, CALT, CEN2, EF-hand protein 2, 20 kD calcium-binding protein, Centrin-2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A_W16110A_PURE_Centrin2_WB_Antibody_081117
Total lysates (15µg protein) from HeLa, MCF-7, 293T and NIH3T3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1mg/mL) purified anti-Centrin 2 antibody (clone W16110A) (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-rat IgG secondary antibody (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder.
  • A_W16110A_PURE_Centrin2_WB_Antibody_081117
    Total lysates (15µg protein) from HeLa, MCF-7, 293T and NIH3T3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1mg/mL) purified anti-Centrin 2 antibody (clone W16110A) (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-rat IgG secondary antibody (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder.
  • B_W16110A_PURE_Centrin2_WB_KOKD_Antibody_081117
    Total lysates (15µg protein) from 293T (Lane 1), 20nM scrambled siRNA (lane 2), 5nM (Lane 3)and 20nM (Lane 4) Centrin 2 siRNA treated 293T cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:1000 diluted (0.5µg/mL) purified anti-Centrin 2 antibody (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-rat-IgG secondary antibody (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower).
  • C_W16110A_PURE_Centrin2_2point5ug_ICC_Antibody_031819
    Purified anti-Centrin 2 (Caltractin) Antibody 293T cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 0 and 1:200 diluted (2.5 μg/ml) anti-Centrin 2 antibody (clone W16110A) overnight at 4 degree followed by 1:200 diluted (2.5 μg/ml) Alexa Fluor® 594 (red) conjugated goat anti-rat IgG (clone Poly4054) incubation for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Cat # Size Price Quantity Check Availability Save
698601 25 µg 81€
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698602 100 µg 203€
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Description

Centrin, also known as caltractin, was first identified in the green alga Tetraselmis striata and is a member of the highly conserved EF-hand superfamily. Centrins are small calcium binding proteins and play essential roles in the duplication and segregation of microtubule organizing centers (MTOCs). All of the three identified human centrins contain four EF-hand motifs and share high amino acid sequence homology. Human Centrin-1 expression is restricted to male germ cells and retina, while Centrin-2 and Centrin-3 are ubiquitously expressed. In addition to its function in centrosome duplication, Centrin-2 has been found to be present in both cytosol and nucleus. Centrin-2 forms a trimeric complex with the human homolog of Rad23B and the xeroderma pigmentosum group C (XPC) protein. The Centrin-2/Rad23B/XPC complex acts as an essential component of the nucleotide excision repair (NER) pathway.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Centrin-2 recombinant protein (full length) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml (1:500 - 1:5000 dilution). For immunocytochemistry, the recommended usage is 0.5 - 2.5 µg per mL (1:100 - 1:500 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Antibody specificity was confirmed by knock-down of Centrin 2 using siRNA (in-house tested)

Product Citations
  1. St-Denis-Bissonnette F, et al. 2023. J Extracell Vesicles. e12387:12. PubMed
  2. Takeda Y, et al. 2024. Nat Commun. 2216:15. PubMed
  3. Stojanovic N, et al. 2024. Sci Rep. 26808:14. PubMed
RRID
AB_2715793 (BioLegend Cat. No. 698601)
AB_2715793 (BioLegend Cat. No. 698602)

Antigen Details

Structure
172 amino acids with a predicted molecular weight of 19.7 kD. Contains four EF-hand domains.
Distribution

Nucleus. Cytoplasm. Centrosome.

Function
Centrin-2 is a calmodulin-related protein involved in the regulation of centrosome duplication and nucleotide excision repair (NER) of DNA.
Interaction
Forms the XPC complex with Rad23B and XPC. Interacts with CCP110 and SFI1.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Antigen References

1. Sawant DB, et al. 2015. Mol Biol Cell. 26:3741.
2. Ying G, et al. 2014. J Neurosci. 34:6377.
3. Nishi R, et al. 2013. Nucleic Acids Res. 41:6917.
4. Boutros R, et al. 2011. Biol Cell. 103:55.
5. Yang CH, et al. 2010. Mol Biol Cell. 21:4361.
6. Nishi R, et al. 2005. Mol Cell Biol. 25:5664.

Gene ID
1069 View all products for this Gene ID
UniProt
View information about Centrin 2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08-10-2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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