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Purified anti-Cofilin 1 Antibody

Pricing & Availability
Clone
A18040J (See other available formats)
Regulatory Status
RUO
Other Names
Cofilin 1, CFL1, CFL
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A18040J_Purified_Cofilin-1_Antibody_1_051019.png
Western blot of purified anti-Cofilin 1 antibody (clone A18040J). Lane 1: Molecular weight marker; Lane 2: 20µg of mouse brain lysate; Lane 3: 40µg of rat brain lysate; Lane 4: 10µg of HeLa cell lysate; Lane 5: 10 µg of NIH3T3 cell lysate. The blots were incubated with 5 µg/mL of clone A8040J or mouse IgG overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18040J_Purified_Cofilin-1_Antibody_1_051019.png
    Western blot of purified anti-Cofilin 1 antibody (clone A18040J). Lane 1: Molecular weight marker; Lane 2: 20µg of mouse brain lysate; Lane 3: 40µg of rat brain lysate; Lane 4: 10µg of HeLa cell lysate; Lane 5: 10 µg of NIH3T3 cell lysate. The blots were incubated with 5 µg/mL of clone A8040J or mouse IgG overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18040J_Purified_Cofilin-1_Antibody_2_051019.png
    IHC staining of purified anti-Cofilin 1 antibody (clone A18040J) on formalin-fixed paraffin-embedded mouse colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A18040J_Purified_Cofilin-1_Antibody_3_051019.png
    IHC staining of purified anti-Cofilin 1 antibody (clone A18040J) on formalin-fixed paraffin-embedded rat colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A18040J_Purified_Cofilin-1_Antibody_4_022020
    ICC staining of purified anti-Cofilin 1 antibody (clone A18040J) on HeLa Cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The cells were co-stained with Flash Phalloidin™ Green 488 (Cat. No. 424201). The slide was mounted with fluoromount G with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
Cat # Size Price Quantity Check Availability Save
865301 25 µg 85€
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865302 100 µg 221€
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Description

Cofilin 1 is a ubiquitously expressed actin-regulating protein that binds to and depolymerizes filamentous actin (F-actin), as well as inhibits G-actin polymerization in a pH-dependent mechanism. Cofilin 1 plays a major role in cell migration, particularly tumor cell migration and invasion. A recent study using colorectal cancer as model, compared expression of cofilin 1 in cancer tissue and adjacent non-tumor tissue and suggested that it may be used as a novel diagnostic tumor marker in various cancers4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CFL1 recombinant protein (a.a. Met 1 - Leu 166)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For WB on brain lysates, 5 - 10 µg/mL is recommended.

RRID
AB_2810771 (BioLegend Cat. No. 865301)
AB_2810771 (BioLegend Cat. No. 865302)

Antigen Details

Structure
Cofilin 1 is a 166 amino acid protein with an apparent molecular mass of ~19 kD.
Distribution

Tissue Distribution: Immune system, gastrointestinal tract, lung, testis, and skin
Cellular Distribution: Nucleus, cytosol, plasma membrane, cytoskeleton, and mitochondrion

Interaction
Cofilin 1 plays a role in cytoskeleton dynamics by regulating F-actin depolimerization
Ligand/Receptor
HSPH1, LIMK1, F-actin
Cell Type
Astrocytes, Dopaminergic Neurons, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area
Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Cell Structure, Neurodegeneration, Neuroscience, Synaptic Biology
Molecular Family
Cytoskeletal Proteins, GTPases, Scaffold Proteins
Antigen References
  1. Kanellos G and Frame MC. 2016. J Cell Sci. 129(17):3211
  2. Walsh KP, et al. 2014. Prion. 8(6):375
  3. Xu P, et al. 2018. Front Plant Sci. 9:1528
  4. Mousavi S, et al. 2018. J Gastrointest Oncol. 9(5):791
Gene ID
1072 View all products for this Gene ID 12631 View all products for this Gene ID 29271 View all products for this Gene ID
UniProt
View information about Cofilin 1 on UniProt.org
Go To Top Version: 2    Revision Date: 02-20-2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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