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Purified anti-CTCF Antibody

Pricing & Availability
Clone
W19193A (See other available formats)
Regulatory Status
RUO
Other Names
CCCTC-Binding Factor, Transcriptional Repressor CTCF, CFAP108
Isotype
Rat IgG2a, κ
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Product Citations
publications
W19193A_PURE_CTCF_Antibody_1_112020
Whole cell extracts (30 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) purified anti-CTCF antibody (clone W19193A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
  • W19193A_PURE_CTCF_Antibody_1_112020
    Whole cell extracts (30 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) purified anti-CTCF antibody (clone W19193A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
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942501 25 µg 95€
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942502 100 µg 235€
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Description

CTCF is an 83 kD transcription factor found in the nucleoplasm, which translocates to the nucleolus when the cell is undergoing cell differentiation. This protein can act as an insulator protein by binding to an unmethylated imprinting control region (ICR) structure and prevent the activation of cis-acting elements which in turn prevents gene activation. CTCF becomes further involved in gene regulation when it forms a complex with cohesin. This complex can stabilize the formation of a chromatin loop, which can promote or repress the binding of other transcription factors to the promoter and enhancer, leading to gene activation or gene repression respectively. CTCF has a role as a tumor suppressor, when the gene goes through a mutation or deletion there is a rise in variance in CpG methylation throughout the genome. This methylation contributes to the development of aggressive and invasive tumors which are seen in breast, endometrial and bladder cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic human CTCF peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 - 2.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC on HeLa cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in high cytosolic background staining.

RRID
AB_2888905 (BioLegend Cat. No. 942501)
AB_2888905 (BioLegend Cat. No. 942502)

Antigen Details

Structure
CTCF is a 727 amino acid protein with a predicted molecular weight of 83 kD.
Distribution

Ubiquitously expressed/nucleus

Function
Transcription factor
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Antigen References

1. Kim S, et al. 2015. Exp Mol Med. 47:e166.
2. Del Rosario BC, et al. 2019. Elife. 8:e42341.
3. Bell AC, et al. 2000. Nature. 405:482-5.

Gene ID
10664 View all products for this Gene ID
UniProt
View information about CTCF on UniProt.org
Go To Top Version: 1    Revision Date: 11-20-2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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